补肾调经方、逍遥丸对促性腺激素预处理小鼠组织蛋白酶-L mRNA的影响  

Effect of Bushen Tiaojing Recipe and Xiaoyao Pill on Expression of Cathepsin-L mRNA in Gonadotropin-primed Mice

作　　者：段彦苍[1] 杜惠兰[1] 贺明[1] 宋翠淼[2] 

机构地区：[1]河北医科大学中西医结合学院,石家庄050091 [2]河北医科大学生理教研室,石家庄050017

出　　处：《中国中西医结合杂志》2011年第1期80-84,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然基金资助项目(No.30772818)

摘　　要：目的探讨补肾调经方、逍遥丸对小鼠组织蛋白酶-L(cathepsin-L,Cat L)mRNA的影响。方法选择未成年小鼠随机分为正常组、对照组、逍遥丸组和补肾调经方组,每组12只,采用反转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)方法检测给予5IU人绒毛促性腺激素(humanchorionic gonadotropin,HCG)0、4、8、12h后Cat L mRNA在各组小鼠卵巢的表达强度。结果注射HCG0、4、8、12h后,Cat L mRNA在促性腺激素预处理小鼠对照组卵巢的表达逐渐升高,Cat L mRNA的相对表达含量分别为:0.066±0.005、0.383±0.045、0.737±0.024、1.036±0.073,各时间点比较差异有统计学意义(P<0.01)。注射HCG后4h与对照组比较,Cat L mRNA逍遥丸组、补肾调经方组小鼠卵巢表达均升高,差异有统计学意义(P<0.01);注射HCG8h与对照组比较,逍遥丸组Cat LmRNA表达升高,差异有统计学意义(P<0.01),补肾调经方组差异无统计学意义(P>0.05);注射HCG12h后与对照组比较,补肾调经方组Cat LmRNA表达升高,差异有统计学意义(P<0.01),逍遥丸组差异无统计学意义(P>0.05)。结论补肾调经方通过使Cat L mRNA表达增强而达到促排卵的作用,消遥丸通过使Cat L mRNA表达高峰提前达到促排卵的目的。Objective To study the effect of Bushen Tiaojing Recipe （BTR） and Xiaoyao Pill （XP）on cathepsin-L （Cat-L） mRNA in mice. Methods Immature mice were randomly divided into the normal group,the control group,the BTR group and the XP group,three in each group. Cat-L mRNA expression in mice was detected using reverse transcription polymerase chain reaction （RT-PCR）at 0,4,8 and 12 h after injecting 5 IU（human chorionic gonadotropin,HCG）. Results Cat-L mRNA expression increased gradually after HCG injection,the relative levels in the control group at 0,4,8 and 12 h were 0.066±0.005,0.383±0.045,0.737±0.024 and 1.036±0.073 respectively,comparisons between different time-points showed significant difference （P0.01）. Compared with the control group,the Cat L mRNA expression was higher at 4 h in both BTR and XP groups（P0.01）,at 8 h in the XP group （P0.05）,and at 12 h in BTR group after injecting HCG （P0.05）.Compared with the control group,cat L mRNA expression showed no statistic difference at 8 h in BTR group and at 12 h in XC group. Conclusions BTR promoted the ovulation by enhancing the expression of CatL gene,and that of XP by advancing the peak of CatL gene expression.

关 键 词：补肾调经方 逍遥丸 排卵 组织蛋白酶-L 反转录聚合酶链式反应 

分 类 号：R285.5[医药卫生—中药学]