柴苓汤对环孢素A肾病大鼠肾小管上皮细胞表型转化的抑制作用  

Inhibitory Effect of Chailing Decoction on Renal Tubular Epithelial Phenotype Transformation in Rats with Cyclosporine A-induced Nephropathy

作　　者：王香婷[1] 魏民[1] 王霞[1] 王筝[2] 王聪慧[2] 孙冬云[1] 许庆友[2] 

机构地区：[1]河北医科大学中医学院,石家庄050091 [2]河北医科大学中西医结合学院,石家庄050091

出　　处：《中国中西医结合杂志》2011年第1期94-98,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：河北省优秀专家出国培训资助项目(No.200892)

摘　　要：目的观察柴苓汤对环孢素A肾病大鼠肾小管上皮细胞表型转化标志物α-平滑肌肌动蛋白(α-smooth muscular actin,α-SMA)的影响,探讨其抑制肾间质纤维化的作用机制。方法 SD大鼠随机分为对照组、模型组、缬沙坦组、柴苓汤组,每组10只。模型组、缬沙坦组及柴苓汤组经口灌服环孢素A[30mg/(kg·d)],复制环孢素A肾病大鼠模型,对照组给予橄榄油;缬沙坦组予缬沙坦[10mg/(kg·d)],柴苓汤组予柴苓汤[3g/(kg·d)],共28天,采用RT-PCR、Western blot、免疫组化、流式细胞术检测大鼠肾脏α-SMA蛋白及mRNA的表达,RT-PCR检测转化生长因子-β1(TGF-β1)和Ⅲ型胶原(ColⅢ)mRNA的表达。结果与对照组比较,模型组大鼠肾脏α-SMA、TGF-β1及ColⅢ表达明显增强(P<0.01或P<0.05),经柴苓汤及缬沙坦治疗,其高表达被显著下调(P<0.01或P<0.05)。结论柴苓汤可通过抑制肾小管上皮细胞的表型转化减缓肾间质纤维化的进程。Objective To observe the effect of Chailing Decoction （CLD） on α-smooth muscular actin （α-SMA,a marker of renal tubular epithelial phenotype transformation） in rats with cyclosporine A （CsA）-induced nephropathy for investigate its mechanism of action in inhibiting tubulo-interstitial fibrosis. Methods Forty Sprague-Dawley rats were equally randomized into four groups：the normal group,the model group,the positive control group and the Chinese medicine（CM） group. Excepting those in the normal group received gastrogavage with olive oil,rats in the other three groups were made into nephropathy model by oral infusion of CsA 30mg/（kg·d） for 28 days. At the same time of modeling,rats in the positive control and CM groups were treated respectively with Valsartan 10 mg/（kg·d） and CLD 3 g/（kg·d）. The mRNA and protein expressions of α-SMA in rats＇ kidney were detected by RT-PCR,Western blot,immunohistochemical and flow cytometry,and the mRNA expressions of transforming growth factor-β1 （TGF-β1） and collagen type Ⅲ （ColⅢ） were determined by RT-PCR. Results Levels of α-SMA,TGF-β1 and Col Ⅲ were significantly higher in the model group than those in the normal group respectively （P0.01 or P0.05）,and the high levels were down-regulated in the positive control and CM groups after treatment （P0.01 or P0.05）. Conclusion CLD could retard the progress of renal interstitial fibrosis by way of inhibiting renal tubular epithelial phenotype transformation.

关 键 词：柴苓汤 环孢素A 肾毒性 Α-平滑肌肌动蛋白 

分 类 号：R285.5[医药卫生—中药学]