消水肿Ⅱ号对妊娠高血压综合征大鼠的防治作用  

The Preventive Effect of XSZⅡ on Pregnancy-Induced Hypertension in Rats

作　　者：孙国珍[1] 陈卫平[2] 史党民[1] 贾淑杰[2] 姚天一[1] 李毅敏[2] 叶娟[1] 王蕾[2] 

机构地区：[1]天津市中心妇产科医院,300052 [2]天津市医药科学研究所

出　　处：《天津医药》2011年第1期62-64,共3页Tianjin Medical Journal

基　　金：天津市卫生局中医;中西医结合科研课题基金(项目编号:07054)

摘　　要：目的:观察消水肿Ⅱ号对亚硝酸左旋精氨酸甲酯(L-NAME)7mg/kg致妊娠高血压综合征(妊高征)大鼠血压、尿蛋白及胎盘胎仔发育的影响。方法:雌雄鼠合笼次日晨,雌鼠阴道涂片发现精子确定为孕0d(E0d),按照顺序依次分入各受试组,即正常孕鼠组(A组,n=6,E10d开始灌胃蒸馏水10mL/kg)、妊高征模型对照组(B组,n=10,E10d灌胃蒸馏水10mL/kg)、硫酸镁阳性对照组(C组,n=8,E14d开始腹腔注射硫酸镁注射液100mg/kg)、消水肿Ⅱ号42g生药/kg组(D组,n=10,E10d开始灌胃消水肿Ⅱ号42g/kg)、消水肿Ⅱ号21g/kg组(E组,n=9,E10d开始灌胃消水肿Ⅱ号21g/kg)。孕期14d开始,除A组外其他各组经静脉注射L-NAME7mg/kg1次/d,共4d。测定大鼠尾动脉血压,尿蛋白含量,胎仔、胎盘质量及死胎率、畸胎率。结果:实验结束时,D组大鼠收缩压,舒张压,平均动脉压与B组比较,差异有统计学意义(均P<0.01)。E组较B组收缩压明显降低(P<0.05)。消水肿Ⅱ号对尿蛋白含量未见明显改善作用。E组死胎率较妊B组明显降低,畸胎率亦显著下降(均P<0.01)。结论:消水肿Ⅱ号能够有效降低妊高征大鼠血压,促进妊高征大鼠胎盘供血和胎仔发育。Objective： To study the hypotensive and preventive effects of XSZⅡ on pregnancy-induced hypertension in rat model established by L-NAME. Methods： Pregnancy rats were assigned to 5 groups according to the sequence of pregnancy including normal pregnancy rats （A group, n = 6, PO distilled water 10 mL/kg from E10）,hypertension control rats （B group, n = 10, PO distilled water 10 mL/kg from E10）,Epsom salt control rats （C group,n = 8, ip Epsom salt 100 mg/kg from E14）,XSZⅡ 42 g/kg group（D group,n = 10, PO XSZⅡ 42 g/kg from 10） and XSZⅡ 21 g/kg group（E group,n = 9, PO XSZⅡ 21 g/kg from E10）. Pregnancy rats were injected 7 mg/kg L-NAME i.v. on the E14 once a day for 4 days except for rats of A group. The values of tail arterial blood pressure, urine protein, placental weight, fetal death rate and birth defects rate were measured in rats. Results： There were different significances in systolic blood pressure, diastolic blood pressure and mean arterial pressure between D and B group（P 0.01）. The systolic blood pressure was significantly decreased in E group compared with that of B group （P 0.05）. There was no significant improvement in urine protein in rats treated by XSZⅡ. The still birth rate was significant lower in E group than that of B group （P 0.01）. The abnormal embryo rate was also significant lower in rats of E group than that of control group （P 0.01）. Conclusion： For pregnancy-induced hypertension, XSZⅡ can effectively reduce the blood pressure and promote blood supply to the placenta and fetal rat development.

关 键 词：高血压 妊娠性 血压 胎盘 死胎 畸胎 疾病模型 动物 大鼠 Sprague-Dawley 消水肿Ⅱ号 

分 类 号：R714.246[医药卫生—妇产科学]