阉割对猪甲状腺转录因子-1、2(TTF-1,2)基因表达的影响  

Influence of Castration on Porcine Thyroid Transcription Factor-1,2(TTF-1,2) Gene Expression

作　　者：王颖[1] 张立凡[1] 蔡兆伟[1] 陈哲[1] 蒋晓玲[1] 周红梅[1] 华绪川[1] 徐宁迎[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029

出　　处：《畜牧兽医学报》2011年第1期1-7,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展规划项目(2006CB102100);国家自然科学基金项目(30972078)

摘　　要：为了研究阉割对甲状腺转录因子-1、2(TTF-1,2)基因的影响,本研究采用荧光定量PCR技术分析阉割与非阉割金华猪甲状腺组织中TTF-1和TTF-2基因在60、90和120日龄时的表达水平。结果表明:非阉割猪TTF-1和TTF-2基因在不同生长阶段的表达量呈稳步上升趋势,阉割猪TTF-1和TTF-2基因的表达基本稳定。TTF-1与TTF-2mRNA的表达量均是在60、90日龄时阉割组高于非阉割组;在120日龄时,阉割组TTF-1与TTF-2mRNA的表达量均低于非阉割组,但差异不显著(P>0.05)。结果,建立的SYBR-Green荧光定量PCR方法可以有效地用于TTF-1和TTF-2基因的表达分析;另外,阉割使得猪甲状腺TTF-1和TTF-2基因在60、90日龄的表达量升高,且TTF-1基因的表达与猪部分肉质性状存在显著的相关性。In order to investigate the effect of castration on expression of TTF-1 and TTF-2 genes,real-time fluorescence quantitative PCR was applied to investigate the developmental expression patterns of TTF-1 and TTF-2 genes from the thyroid tissue of castrated and uncastrated Jinhua pigs on the age of 60,90 and 120 days.The results showed that the expression of TTF-1 and TTF-2 genes increased slightly in uncastrated pigs,while they were relatively stable in castrated pigs.The expression level of TTF-1 and TTF-2 mRNA in castrated Jinhua pigs on 60 and 90 days were higher than that of uncastrated pigs,while the expression level of TTF-1 and TTF-2 on 120 days were lower than that of uncastrated pigs,but no significant difference（P0.05）.The results indicated that the real-time PCR method was faithful for detecting the expression analysis of TTF-1 and TTF-2 genes,and the expression of TTF-1 and TTF-2 mRNA increased after castration.Furthermore,there was a significant correlation between TTF-1 gene expression and meat quality traits.

关 键 词：猪 阉割 荧光定量PCR 甲状腺转录因子-1(TTF-1) 甲状腺转录因子-2(TTF-2) 

分 类 号：S828[农业科学—畜牧学] Q344.13[农业科学—畜牧兽医]