隐性乳房炎奶牛IL-8受体与乳铁蛋白基因的PCR-SSCP分析  

Analysis on the IL-8 Receptor and Lactoferrin Genes in Subclinical Mastitics-dairy Cows by PCR-SSCP

作　　者：何高明[1] 张素华[1] 赵宗胜[1] 李大全[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《畜牧兽医学报》2011年第1期136-140,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：石河子大学科学研究发展项目"荷斯坦奶牛隐性乳腺炎相关基因分型与致病菌的研究"(2006)

摘　　要：本研究旨在探讨IL-8受体基因、乳铁蛋白基因多态性与奶牛乳房炎的相互关系。试验采用PCR-SSCP技术分别对80头健康奶牛和隐性乳房炎奶牛的IL-8受体基因和乳铁蛋白基因进行了多态性检测,并分析了它们与奶牛乳汁中体细胞数的关系。PCR-SSCP分析结果表明,IL-8受体CXCR1基因SSCP-5、SSCP-7在健康奶牛个体中出现程度较高,说明其个体对隐性乳房炎表现抗性;在乳铁蛋白基因5′调控区的不同基因型中,AB基因型个体对奶牛隐性乳房炎表现抗性。The aim of this study was to detect the relationships between the genotypes of IL-8R and Lactoferrin genes and subclinical mastitics.The genotypes of IL-8R and Lactoferrin genes of 80 healthy and 80 subclinical mastitics-dairy cows were detected by using PCR-SSCP technique,and the relationships with the number of somatic cells in the milk were analysed.The results showed that the frequencies of genotype SSCP-5 and SSCP-7 of IL-8R CXCR1 were high in the healthy dairy cows and indicated that individuals with genotype AB performed resistance to subclinical-mastitics.

关 键 词：隐性乳房炎 IL-8受体基因 乳铁蛋白基因 PCR-SSCP 

分 类 号：S823.91[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]