Asia 1型FMDV感染BHK-21细胞差异蛋白质点2-DE分析  

Two-dimentional Gel Electrophoresis Analysis of Differential Protein Spots from BHK-21 Cell Infected FMDV Serotype Asia 1

作　　者：刘永杰[1] 张克山[1] 尚佑军[1] 郭建宏[1] 郑海学[1] 田宏[1] 卢国栋[1] 刘湘涛[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,国家口蹄疫参考实验室,兰州730046

出　　处：《畜牧兽医学报》2011年第1期145-149,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十一五”国家支撑计划课题(2006BAD06A03);国家现代肉羊产业技术体系(nycytx-39)

摘　　要：为研究Asia 1型口蹄疫病毒(FMDV)感染BHK-21细胞后蛋白质组的变化及差异,将FMDV接种于培养的单层BHK-21细胞,同时设未接毒的BHK-21细胞作对照组。待细胞病变后分别提取2组细胞总蛋白借助2-DE技术及PDQuest8.0软件进行细胞蛋白质组二维电泳图谱差异性分析。结果显示感染组有1 457个可见蛋白质点,而对照组可见的蛋白质点为1 427个。感染组蛋白点的表达量与对照组相比,二者具有统计学差异(P<0.05)的蛋白质点472个,其中表达上调251个点,表达下调221个点,新合成蛋白点30个。结果表明FMDV感染BHK-21细胞后引起了细胞蛋白质组表达模式的改变,这种变化是病毒与细胞相互作用的结果。本研究首次利用蛋白质组学技术研究Asia 1型FMDV感染BHK-21细胞后蛋白质组学差异,有助于深入研究Asia 1型FMDV和BHK-21细胞相互作用的分子机制。To examine the differences and changes of proteome of BHK-21 cells after infection with foot-and-mouth disease virus（FMDV） serotype Asia 1,the monolayer BHK-21 cells infected with FMDV and normal cells were prepared as experimental materials.The total protein from the BHK-21 cells were isolated and used to analyze the proteome differences between the infected and the control cells by 2-dimentional gel electrophoresis（2-DE）.Results showed that there were 1 457 protein spots visible in the infected cells,and 1 427 visible protein spots in the control cells.Compared with control cells,there were significance of statistics（P0.05） at 472 differential protein spots in infection cells,including 251 up-expressed protein spots,221 down-expressed protein spots and 30 newly proteins spots.These results indicate that the expression pattern of cells are changed after infection with FMDV.It maybe results from inter-actions between viral and cellular components.This is the first time to use proteomics technologies to study the proteome differences after FMDV infection and this study is helpful to further research about molecular mechanisms of interactions between FMDV serotype Asia 1 and BHK-21 cells.

关 键 词：Asia1型FMDV BHK-21细胞 感染 差异蛋白质组 

分 类 号：S852.659.6[农业科学—基础兽医学]