应用原子力显微镜分析猪脂肪前体细胞的分化  

Analysis of porcine preadipocytes differentiation by atomic force microscope

作　　者：李盛璞[1] 师如意[2] 王秋兰[1] 王牡[1] 甘睿[3] 潘洁[1] 蔡继业[1] 张守全[2] 

机构地区：[1]暨南大学化学系,广州510632 [2]华南农业大学动物科学学院,广州510642 [3]暨南大学药学院,广州510632

出　　处：《生物工程学报》2011年第1期124-130,共7页Chinese Journal of Biotechnology

基　　金：国家重点基础研究发展计划(973计划)(No.2010CB833603);国家自然科学基金(No.30872404)资助~~

摘　　要：脂肪形成过程中发生的异常变化与许多疾病的产生有着密切的关系。为深入了解脂肪形成的机制,利用原子力显微镜研究脂肪前体细胞向成熟脂肪细胞分化前后细胞形貌、超微结构和机械性能的变化。结果表明,脂肪前体细胞与成熟脂肪细胞在形貌上存在明显的差异。在超微结构的探测中成熟脂肪细胞表面粗糙度低于脂肪前体细胞。通过力曲线的分析得出,分化前后两种细胞的机械性质均发生改变。脂肪前体细胞在粘弹力、硬度和杨氏模量的研究中比成熟脂肪细胞都高出约20%。原子力显微镜在纳米尺度上分析脂肪前体细胞向成熟脂肪细胞分化过程中细胞膜的改变,其研究结果为进一步探讨脂肪形成机制提供可视化定量数据。Abnormal changes during fat formation are closely related to the prevalence of many diseases.In order to understand the formation mechanism of fat,we used atomic force microscopy（AFM） to characterize the morphology and mechanical properties of porcine preadipocytes during the differentiation.Preadipocytes and adipocytes were different morphologically.The surface roughness of adipocytes was less than preadipocytes by detection of the ultrastructure.The mechanical properties of preadipocytes were changed during differentiation with AFM-based force spectroscopy.Preadipocytes were 20% higher than adipocytes in the adhesion force,stiffness and Young＇s modulus.Therefore,AFM analysis of membrane changes related to adipocytes formation provided quantitative data in the nanometer level for further studying the formation mechanism of the adipocytes.

关 键 词：原子力显微镜 脂肪前体细胞 成熟脂肪细胞 超微结构 机械性能 

分 类 号：Q591.5[生物学—生物化学]