百草枯诱导PC12细胞损害和miR-133b表达的变化  

Induction of cell damage and change of miR-133b expression by paraquat in PC12 cells

作　　者：李煌元[1] 吴思英[2] 

机构地区：[1]福建医科大学公共卫生学院,福建省重点建设大学环境与健康重点学科,环境与健康研究所毒理学与化学物安全性评价研究室,职业与环境卫生学系 福州,350004 [2]福建医科大学公共卫生学院,福建省重点建设大学环境与健康重点学科,环境与健康研究所毒理学与化学物安全性评价研究室公共卫生学院流行病与卫生统计学系, 福州,350004

出　　处：《中华劳动卫生职业病杂志》2011年第1期2-6,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：基金项目：国家自然科学基金资助项目（30800936）

摘　　要：目的探讨百草枯（P0）对大鼠肾上腺嗜铬细胞瘤（PC12）细胞毒性作用和对miR-133b表达的影响。方法以50、100、300μmol／LPQ分别处理PCI2细胞24h，用噻唑蓝（MTT）法检测PCI2细胞毒性；以100、300μmol／LP0分别处理PCI2细胞24h，用AnnexinV．FITC／PI法流式细胞仪检测细胞凋亡，用荧光定量反转录聚合酶链反应（RT—PCR）法检测miR-133b相对表达水平。结果与对照组比较，100、300μmol／LPQ处理细胞24h后细胞存活率明显下降，差异有统计学意义（P〈0．05或P〈0．01）。与对照组比较，100、300μmol／LPQ处理细胞24h后细胞凋亡率明显升高，差异有统计学意义（P〈0．01）。300μmol／LPQ处理细胞24h可诱导细胞miR-133b表达，差异有统计学意义（P〈0．05）.结论PQ致PCI2细胞损害和细胞凋亡时miR-133b的表达上调。Objective To investigate the effect of paraquat on induction of cell damage and miR-133b expression in PC 12 ceils. Methods Cytoyoxicity of PC 12 cells was measured by MTT assay, following the PC 12 cells treatment with 50,100, or 300 μmol/L paraquat. Cell apoptosis was examined by the method of Annexin V-FITC/PI in flow cytometry （FCM） and the relative level of miR-133b expression was measured by real time RT-PCR, following the PC12 cells treatinent with 100 or 300 μmol/L paraquat. Results Survival rate of PC12 cells treated with 100 or 300 μmol/L paraquat was lower than that of the vehicle control group （P〈0.01, P〈0.05）, in the dose dependent pattens. Apoptotic rate of PC12 cells treated with 100, 300 μmol/L paraquat was higher than that of the vehicle control group （/）〈0.05）. The relative level of miR- 133b expression of PC 12 ceils treated with 300 μmol/L paraquat was higher than that of the vehicle control group （P〈0.05）. Conclusions Paraquat may cause cell damage and induce apoptosis in PC12 cells, and induce miR-133b expression.

关 键 词：百草枯 细胞毒性试验 PC12细胞 基因表达 

分 类 号：R5[医药卫生—内科学]