高分辨率熔解曲线技术对苯作业人员MDR1C3435T检测的应用  

High-resolution melting： a analysis for gcnotyping of MDR1 C3435T in benzene-exposed workers

作　　者：黄简抒[1,3] 张心菊[2] 许笑[2] 关明[2] 周元陵[1] 吕玲[3] 邹和建[3] 

机构地区：[1]复旦大学附属金山医院职业病科,上海200540 [2]复旦大学附属华山医院中心实验室,上海200540 [3]复旦大学附属华山医院职业病科,上海200540

出　　处：《中华劳动卫生职业病杂志》2011年第1期70-72,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：基金项目：上海市金山区科委科研项目（2009-3-19）

摘　　要：目的探讨利用高分辨率熔解曲线技术（HRM）检测苯作业人员MDR1C3435T的可行性。方法首先应用聚合酶链反应-限制性片段长度多态性分析（PCR—RFLP）检测121例苯作业人员MDR13435的基因型，再随机抽取10％样奉进行HRM分析，在进行HRM的样本中随机抽取6例进行测序验证，并对HRM、PCR—RFLP的优劣性进行比较。结果随机抽取的10％样本经HRM分析后与PCR—RFLP检测的基因型完全一致，经测序证实酶切前PCR产物为244bp，同时证实MDR，3435位点存在C／C型、C/T型、T/T型3种基因型，两种疗法均可靠。HRM在检测MDR1C3435T时比RFLP具有耗时短、易于操作、价格便宜的优点。结论HRM是理想的DNA诊断方法，适合在职业健康监护中筛查易感基因。Objective Using high resolution tnehing （HRM） to analysis MDR1 C3435T in people exposed to benzene. Methods Restriction fi＇agment length polymorphism （RFLP） was utilized to detect the polymorphism of MDR1 3435 in 121 benzene-exposed workers, and the results were comtbared with the HRM in 10% samples and were confirmed with direct sequencing for six people in them. Results By direct sequencing, consistent results of benzene-exposed workers with RFLP or HRM were got. The new high resolution melting curve analysis is more efficient, more convenient, and cheaper than RFLP. Conclusion High-resolu- tion melting analysis provides a valid approach to efficiently detect DNA genetic diagnosis, which is suitable for detect susceptible genes in occupational surveillance.

关 键 词：苯 基因表达型 多态性 单核苷酸 基因携带体检测 

分 类 号：R440[医药卫生—诊断学]