CVB3腺病毒载体Ad/sVP1-C3d3的构建及免疫效果研究  

Construction of the recombinant adenovirus expressing the CVB3 sVP1-C3d3 protein and study on its immunological effects in mice

作　　者：李嘉[1] 李剑[1] 羡鲜[1] 刘贵霞[1] 蓝佳明[1] 金玉怀[1] 谢立新[1] 张桓[1] 王永祥[1] 

机构地区：[1]河北医科大学病原生物学教研室,河北石家庄050017

出　　处：《细胞与分子免疫学杂志》2011年第1期47-50,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省科学技术研究与发展计划(08276101D-93)

摘　　要：目的:构建柯萨奇病毒B3(CVB3)VP1基因重组腺病毒Ad/sVP1-C3d3,并观察对小鼠的免疫效果。方法:利用AdEasy-1系统构建、包装重组腺病毒Ad/sVP1-C3d3。BALB/c小鼠随机分为Ad/sVP1-C3d3、Ad/VP1、Ad和PBS4组,肌肉注射免疫,共免疫2次,每次间隔16d。用ELISA法和微量中和试验法分别检测血清CVB3VP1IgG和中和抗体滴度;CCK-8法检测特异性CTL杀伤活性;用致死量CVB3攻击小鼠后,检测血中病毒滴度。结果:成功构建、包装了重组腺病毒Ad/sVP1-C3d3。免疫小鼠后,Ad/sVP1-C3d3组血清CVB3VP1IgG滴度和中和抗体水平明显高于Ad/VP1(P<0.01),CTL杀伤活性明显高于Ad和PBS对照组(P<0.01)及Ad/VP1组(P<0.05),血清病毒滴度低于Ad和PBS对照组(P<0.05)。结论:重组腺病毒Ad/sVP1-C3d3能明显提高小鼠的细胞和体液免疫应答并降低血液病毒载量。AIM： To construct recombinant adenovirus Ad/C3d3-sVP1 and investigate the immune effects against coxsackievirus infection in mouse. METHODS： The recombinant adenovirus Ad/sVP1-C3d3 was constructed and packaged. BALB/c mouse were divided into four groups： Ad/sVP1-C3d3 group, Ad/VPI group, Ad group and PBS group. The mice in each group were immunized by intramuscular injection. The titers of sera IgG and neutralizing antibody were detected by ELISA method and trace neutralization assay, respectively.

关 键 词：柯萨奇病毒B3 腺病毒载体 小鼠 

分 类 号：R392.12[医药卫生—免疫学]