不同手术方式对幼兔鼻翼软骨发育影响的实验研究  

Influence of different Surgeries on growth and development of alar cartilage in young-rabbit

作　　者：蒋练[1] 董茜茜[1] 宋庆高[1] 陈尚[1] 邹四海[1] 何苇[1] 

机构地区：[1]遵义医学院附属口腔医院口腔颌面外科,贵州遵义563000

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第2期74-77,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：贵州省卫生厅资助(No:D-255);遵义医学院中青年基金资助(No:F-178)

摘　　要：目的:观察不同手术方式对幼兔鼻翼软骨生长发育的影响,为鼻畸形修复术式选择奠定一定基础。方法:健康新西兰幼兔随机分为3组,空白对照组4只,实验组Ⅰ和实验组Ⅱ各12只,实验组Ⅰ、Ⅱ幼兔分别进行鼻翼弧形切口下的潜行剥离及宽度为2 mm×2 mm鼻翼软骨切除。分别于术后4、8、12、16周处死幼兔,完整取出鼻翼软骨,进行组织学及免疫组织化学分析。结果:生长发育各阶段潜性剥离实验侧和对照侧鼻翼软骨细胞生长的各个指标比较差异无统计学意义,两侧软骨细胞的增殖活性一致;而鼻翼软骨部分切除组实验侧断端未形成软骨组织修复,甚至在个别切片上见到有鼻腔黏膜组织长入缺损空隙。结论:潜性剥离鼻翼软骨所造成的创伤对幼兔鼻翼软骨的发育没有不良影响,术后可以形成一个发育成熟的完整个体,而早期切断鼻翼软骨对鼻翼软骨的发育有不良影响,潜在的透明软骨修复能力在生长发育过程中无法完全代偿手术本身的损伤。Objective：The purpose of this study is to observe the effection of different clinical surgeries on alar nasal cartilages＇growth and development.The experimental results can provide some theory basis for clinical surgeries.Method：Twenty-eight New Zealand immature rabbits were used in this study,and divided into normal control group,hidden dissection group and cutting off alar nasal cartilages group randomly,which included 4,12 and 12 rabbits,seperately.Arc incision were made on the mucous membrane of nasal cavity,and then dissect the alar nasal cartilages hiddenly or cut off the alar nasal cartilages,seperately.The growth and development of the alar cartilage were observed at different stages after the surgery using histological and immuno-histochemical methods.Result：Four weeks,eight weeks,twelve weeks and sixteen weeks after surgery,there were no significant differences in the indexes of chondrocytes between hidden dissection group and control group.In cutting off alar nasal cartilages group,fiber tissue were observed in the vacancy left after being cut off cartilages,and even mucous membrane tissue could be seen in some slices.Conclusion：There is no adverse influence on the growth and development of the alar cartilage after being hidden dissected.Contrarily,the restoring capability of transparent cartilage cannot counteract the jnjury resulted form the surgery after the alar nasal cartilages being cut off.

关 键 词：潜性分离 切断鼻翼软骨 鼻翼软骨 生长发育 

分 类 号：R246.8[医药卫生—针灸推拿学]