猪急性心肌梗死模型cTnI与DE-MRI相关性实验研究  

Detection of the Serum Cardiac Troponin I(cTnI) in Swine Models with Acute Myocardial Infarction:a Correlative Study with Delayed-enhancment MR Imaging

作　　者：赵新湘[1] 杨达宽[2] 袁曙光[1] 杨宣涛[3] 杨新焕[1] 郝建成[1] 

机构地区：[1]昆明医学院第二附属医院放射科MRI室,云南昆明650101 [2]昆明医学院第二附属医院心胸外科,云南昆明650101 [3]云南省第一人民医院病理科

出　　处：《临床放射学杂志》2011年第1期106-110,共5页Journal of Clinical Radiology

基　　金：云南省科技厅联合专项基金资助项目(基金编号2007C0015R)

摘　　要：目的探讨临床试剂盒是否适用于猪心肌梗死模型心肌肌钙蛋白(cTnI)的检测,其检测的cTnI峰值与DE-MRI所检测心肌梗死积分的关系。材料与方法 10只制备成功的猪急性心肌梗死模型分别于术前、术后1h、2h、5h、24h、48h、72h、1周采血行cTnI检测,所用试剂盒为临床试剂盒;术后行延迟增强MRI(DE-MRI)检查,心脏标本行氯化三苯基四氮唑(TTC)染色和免疫组织化学检查。结果 10例TTC染色和免疫组织化学均证实有心肌梗死;10例cTnI术后1h可见上升,于24h达高峰,24h峰值与DE-MRI、TTC检测的心肌梗死积分高度相关,相关系数分别为0.84、0.855(P值<0.05),有显著统计学意义。结论临床试剂盒对猪血清cTnI术前及术后检测,可反映猪心肌损伤;其24h峰值与DE-MRI心肌梗死范围强相关;两者均可活体内检测,且对坏死心肌有高度敏感性,两者结合有助于结果的相互印证和互为补充,可更准确地评价心肌梗死。Objective To investigate the relationships of the serum cardiac troponin I（cTnI）peak value with quantitative grade by delayed-enhancment MR imaging（DE MRI）in swine model with acute myocardial infarction.Materials and Methods In Ten swine models with acute myocardial infarction were collected in the study,cTnI were acquired before operation and at 1h,2h,5h,24h,72h,and 1w after operation.The swine models also underwent DE MRI after operation.The heart samples were TTC stained and were performed immunohistochemistry examination.Results Ten cases of myocardial infarction were proved by immunohistochemistry examination and TTC staining.Serum cTnI elevated at 1h after operation,and peaked at 24 h after operation.Peak value of cTnI was correlated strongly with the quantitative grade of myocardial infarction assessed by TTC（correlation coefficien t=0.85,P=0.002） and by DE MRI（correlation coefficien t=0.84,P=0.002）.Conclusion The serum cardiac troponin I combined with DE MRI is applicable to evaluate myocardial infarction caused by coronary heart disease.

关 键 词：心肌梗死 猪 肌钙蛋白 延迟增强 磁共振成像 

分 类 号：R542.22[医药卫生—心血管疾病]