TNF-α和IL-2在肝移植急性排斥反应中的表达及意义  

TNF-α and IL-2 expression and significance in acute rejection of liver transplantation

作　　者：樊斌[1] 李德卫[1] 冯渊[1] 杨晓波[1] 

机构地区：[1]重庆医科大学附属第一医院肝胆外科,重庆400016

出　　处：《中国普通外科杂志》2011年第1期11-14,共4页China Journal of General Surgery

摘　　要：目的检测肿瘤坏死因子α(TNF-α)和白细胞介素2(IL-2)在肝移植术后急性排斥反应中的表达情况,以评价其作为肝移植术后急性排斥反应早期诊断指标的价值。方法建立大鼠原位肝移植模型,应用荧光定量PCR法检测大鼠肝移植术后肝组织中TNF-α和IL-2基因的表达,以组织病理学作为急性排斥反应的诊断标准,研究其表达与急性排斥反应的关系。结果术后3,5,7d异基因肝移植大鼠移植肝均有急性排斥反应发生,其肝组织TNF-α及IL-2表达水平均显著高于同期同基因肝移植大鼠肝组织的TNF-α,IL-2表达水平。结论 TNF-α和IL-2参与肝移植后排斥反应的发生,其表达可作为术后急性排斥反应的辅助诊断指标。[中国普通外科杂志,2011,20(1):11-14]Objective To investigate the expression of TNF-α and IL-2 in acute rejection of liver transplantation and evaluate their value as predictive indicators of acute rejection.Methods After orthotopic liver transplantation models was established in rats,real-time fluorescent quantitative reverse transcriptase polymerase chain reaction（RT-PCR） was used to detect the gene expression of TNF-α and IL-2 in the transplanted liver.The relationship between TNF-α/IL-2 expression and acute rejection was analyzed based on histopathological standard of acute rejection.Results On day 3,5 and 7 after transplantation,acute rejection occurred in the graft of all allogenic transplantation rats,and the mean levels of TNF-α and IL-2 in liver tissues were significantly higher in allogenic transplantation group than those in syngenic transplantation group.Conclusions TNF-α and IL-2 expression are related to the development of acute rejection after liver transplantation,and may be used as sensitive and reliable accessory indicators in detecting acute rejection.

关 键 词：肝移植 TNF-Α IL-2 排斥反应 

分 类 号：R617[医药卫生—外科学]