白术多糖对缺血再灌注损伤大鼠肝脏的保护作用  

The protective effect of atractylodes macrocephalaon polysaccharide on hepatic ischemia reperfusion injury in rats

作　　者：张培建[1] 张杰[1] 卜平[1] 刘歆农[1] 苏辉[1] 孟中良[1] 庄卓男[1] 伍学艳[1] 

机构地区：[1]扬州大学医学院普通外科研究室,江苏扬州225000

出　　处：《中国普通外科杂志》2011年第1期62-66,共5页China Journal of General Surgery

基　　金：江苏省卫生厅面上科研课题(H200770);江苏省中医药科技项目(LZ09133)

摘　　要：目的探讨白术多糖对大鼠肝脏缺血再灌注(I/R)损伤的保护作用。方法成年雄性SD大鼠随机分成3组:假手术组,I/R组,白术多糖预处理缺血再灌注组(PC组),分别于成模后1,6,24 h 3个时段分别检测3组大鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST)水平;检测肝组织中ICAM-1 mRNA含量及IL-1的表达。结果 PC组及I/R组ALT,AST水平以及ICAM-1 mRNA含量和IL-1表达均高于假手术组(P<0.05),但PC组上述4个指标均较I/R组显著降低,差异有统计学意义(P<0.05)。结论白术多糖可能通过降低ICAM-1的含量而减少炎症因子IL-1的生成,从而对肝脏I/R损伤起保护作用。Objective To investigate the protective effect of atractylodes macrocephalaon polysaccharide on ischemia-reperfusion（I/R）injury of liver in rats.Methods A total of 54 male SD rats were randomly divided into 3 groups： sham group,I/R group and preconditional treatment of atractylodes macrocephalaon polysaccharide group（Group PC）.The rats were killed at 1,6,24h after 60 mins of models conducted,and the serum ALT and AST level,the expression of ICAM-1 mRNA and IL-1 in liver tissue were observed.Results Compared with sham group,the ALT and AST levels,the expression of ICAM-1 mRNA and IL-1 were significantly increased in PC and I/R groups,but compared to I/R group,the ALT and AST levels and the expression of ICAM-1 mRNA and IL-1 in PC group were significantly decreased（P0.05）.Conclusions Atractylodes macrocephalaon polysaccharide may have protection to ischemic injury of rat liver through reduction of ICAM-1 expression and decrease production of IL-1.

关 键 词：再灌注损伤 肝 白术多糖/治疗应用 大鼠 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学]