传统针刺助产处方对孕晚期大鼠子宫收缩影响的电针参数优化研究  

Effects of electroacupuncture at points of a traditional acupuncture formula that induces labor with different parameters on uterine contractility in late-stage pregnant rats

作　　者：陈滢如[1] 苑鸿雯[1] 舒福政[1] 任晓暄[1] 马良宵[1] 刘仁权[2] 朱江[1,3] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京中医药大学信息中心,北京100029 [3]国家中医药管理局针灸特色疗法评价重点研究室,北京100029

出　　处：《中西医结合学报》2011年第1期91-99,共9页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金面上项目资助( No .30973793);国家重点基础研究发展计划(973计划)资助项目( No .2006CB504503)

摘　　要：目的:通过比较不同电针参数组合传统针刺助产处方合谷-三阴交穴对孕晚期大鼠子宫收缩的影响,筛选该处方最佳电针参数组合,为提高助产效果、增强可操作性以及进一步的机制研究提供依据。方法:选用健康成年雌性Wistar大鼠108只,分批次随机分为空白对照组与妊娠组。妊娠组大鼠采用自由合笼托盘阴栓观察法制备妊娠模型,受孕后随机分为6个电针组(应用2×3析因设计,频率刺激方式为连续波、疏密波,频率选择15、30、50Hz)和妊娠模型组。电针组均先电针双侧合谷穴20min再加刺双侧三阴交穴5min,共针刺25min,电针的同一对电极一端连接穴位,另一端连接穴位旁开2～3mm近心端的非穴点,电流强度0.2～0.3mA。于大鼠子宫下段连接肌肉张力换能器及BL-420生物机能实验系统,记录大鼠子宫收缩张力变化幅度、收缩频率及收缩波时间。结果:与空白组比较,模型组及电针组的子宫收缩张力变化幅度、收缩频率及收缩波时间均显著增加(P<0.01或P<0.05)。与模型组比较,电针1、6组的子宫收缩张力变化幅度显著增加(P<0.01或P<0.05);电针6组子宫收缩频率显著增加(P<0.01或P<0.05);电针1、5、6组子宫收缩波时间均显著增加(P<0.01或P<0.05)。电针6组与各电针组比较,除电针1组外,电针6组子宫收缩张力变化幅度、子宫收缩频率较其他电针组显著增加(P<0.01或P<0.05),电针6组与电针1组之间比较,差异无统计学意义(P>0.05),但电针6组均值均大于电针1组;除电针1组和5组外,电针6组子宫收缩波时间较其他电针组均显著增加(P<0.01或P<0.05),电针6组与电针1、5组之间差异无统计学意义(P>0.05),但电针6组均值均大于电针1组和5组。结论:2Hz和50Hz交替的疏密波(电针6组)的组合方式可显著增加孕晚期大鼠子宫收缩张力变化幅度、收缩频率及收缩波时间,建议作为传统针刺助产处方的电针参数推荐组合方式,并建议�Objective：To select the most effective parameters of electroacupuncture （EA） at Hegu （LI4） and Sanyinjiao （SP6）,a traditional acupuncture formula that induces labor,by comparing its effects on uterine contraction in late-stage pregnant rats,so as to improve the effects of acupuncture on induction of labor and its clinical maneuverability and provide the basis for further mechanism research.Methods：One hundred and eight female healthy adult Wistar rats were randomly divided into eight groups including one non-pregnancy control group （normal control group） and seven pregnant groups. Pregnant rats were prepared by free mating and vaginal plugs observation method and were randomly divided into six EA groups and one no EA group （model group） according to the requirements of a 2×3 factorial design. EA parameters included 2 types of waves （continuous wave and sparse-dense wave） and 3 types of frequency （15,30 and 50 Hz）. Rats in treatment groups received EA on bilateral LI4 for 20 min and then bilateral SP6 for 5 min with certain parameters accordingly. Each acupoint and a nearby non-acupoint at the proximal end （2 to 3 mm from the acupoint） were connected with two leads of one EA line. The current intensity of EA was 0.2 to 0.3 mA. A muscle tension transducer and the BL-420 Biological Experimental System were placed on the lower uterine segment of rats for detecting contractility,frequency and lasting-time of contraction wave.Results：Compared with non-pregnancy control group,the amplitude,frequency and lasting time of contraction waves of uterine contraction in all EA groups and model group were significantly increased. Compared with control group,amplitudes of contraction waves of uterine contraction of both EA1 group （15 Hz continuous wave） and EA6 group （2/50 Hz sparse-dense wave） were significantly increased （P〈0.01 or P〈0.05）; frequency of contraction waves of uterine contraction of EA6 group was significantly increased （P〈0.01 or P〈0.05）; lasting time

关 键 词：电针 分娩 子宫收缩 合谷穴 三阴交穴 大鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]