缺血预处理对大鼠肺缺血再灌注肺损伤的保护作用  

Protective function of ischemia preconditioning on rat lung ischemia-reperfusion injury

作　　者：万小蔹[1] 朱建荣[1] 王红梅[1] 黄丽蓉[1] 庄少侠[1] 

机构地区：[1]无锡市人民医院胸外科,江苏无锡214023

出　　处：《山东大学学报（医学版）》2011年第1期28-30,38,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨缺血预处理对大鼠肺缺血再灌注肺损伤的保护作用及其机制。方法将清洁级SD大鼠36只随机分为假手术组(Sham组)、缺血再灌注组(I/R组)、缺血预适应+缺血再灌注组(IP+I/R组),各12只,分别进行相应处理。观察肺组织病理变化及细胞凋亡情况,测量肺组织含水量;检测血清及肺组织匀浆中超氧化物歧化酶(SOD)活力、丙二醛(MDA)及内皮素-1(ET-1)的浓度。结果与Sham组比较,I/R组光镜下见肺组织病变明显加重,细胞凋亡数量明显增加,肺组织含水量明显增加,血清及肺组织中SOD活力明显降低,MDA及ET-1的浓度明显增高(P<0.01);与I/R组比较,IP+I/R组肺组织病变减轻,细胞凋亡数量明显减少,肺组织含水量明显降低,血清及肺组织中SOD活力明显升高,MDA及ET-1的浓度明显降低(P<0.01)。结论缺血预适应对缺血再灌注肺损伤具有保护作用,其作用机制可能与激活内源性抗氧化作用、灭活或减少氧自由基、保护肺脏血管内皮的损伤有关。Objective To explore the protective function and mechanism of ischemia preconditioning on rat lung ischemia-reperfusion injury, Methods 36 SD rats were randomly divided into the control group （Sham groups）, the ischemic reperfusion group （I/R group）, and the ischemia preconditioning ＋ ischemic reperfusion group （IP ＋ I/R）, 12 rats in each group. Lung tissue and cell apoptosis pathological changes were observed, lung tissue moisture was meas- ured, and serum and lung homogenate super oxide dismutase SOD vigor and malondialdehyde （MDA） and endothelin- 1 （ET-1） concentrations were determined. Results Compared with the sham groups, the pathological changes and the number of apoptosis and water content of lung tissues in the I/R group were significantly increased （ P 〈 0.01 ）, the serum and lung tissue SOD activity were significantly decreased, and MDA and concentration of ET -1 also were signifi- cantly increased （ P 〈 0.01 ）. Compared with the I/R group, pathological changes and the number of apoptosis and wa- ter content of lung tissue in the IP ＋ I/R group were significantly decreased （P 〈0.01 ）, serum and lung tissue SOD activity were significantly increased, and MDA and concentration of ET -1 were significantly decreased （P 〈 0.01 ）. Conclusion Ischemic preconditioning may protect ischemia-reperfusion lung injury in rats, which may be related to activation of endogenous antioxidant inactivation or reduction of oxygen free radicals.

关 键 词：缺血预处理 大鼠 缺血再灌注 肺损伤 

分 类 号：R655.3[医药卫生—外科学]