嗜酸性粒细胞增多症转基因小鼠模型的病理学观察  

Eosinophilia in a transgenic mouse model:a pathological study

作　　者：张敏[1] 亓翠玲[1] 王会萍[1] 叶志金[1] 唐富天[1] 王尽淘[1] 耿建国[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广州510006

出　　处：《临床与实验病理学杂志》2011年第1期75-78,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金资助项目(30871304);国家科技部"973"项目(国家重点基础研究发展计划2010CB529702)

摘　　要：目的研究过表达IL-5的嗜酸性粒细胞增多症转基因小鼠模型的生物学特性和病理学变化。方法记录转基因小鼠的繁育情况,通过计数小鼠外周血中嗜酸性粒细胞的变化规律;观察嗜酸性粒细胞增多对器官损害的病理变化特征。结果小鼠已成功繁育,外周血中嗜酸性粒细胞占白细胞数的比例持续增长到48周龄时的49.9±3.5%,脏器组织内出现嗜酸性粒细胞浸润和组织细胞变性、坏死等病理改变,出现体表被毛缺失和直肠脱垂现象。结论小鼠体内高水平的IL-5能显著影响外周血内嗜酸性粒细胞的数目和比值,造成了肺脏、肝脏和脾脏等脏器的严重损坏。表明该小鼠是研究嗜酸性粒细胞增多症良好的转基因小鼠模型。Purpose To investigate the biological features and pathologic changes of spontaneous eosinophilia in a transgenic mouse model.Methods The reproduction of the transgenic mouse was recorded.The proportion of eosinophils in whole peripheral white blood cells was determined.Other pathological processes such as organ injuries caused by eosinophilic infiltration were also investigated.Results N.J.1638 mice were raised well in our lab.The proportion of peripheral eosinophils in whole white blood cells increased from low level at birth to 49.9% at 48 weeks old.Eosinophils deposited in the lung,liver,spleen and other organs and caused pathological changes such as cell degeneration,necrosis and fibrous hyperplasia.When aged,the mice developed the dermal phenotype of hair loss.Some mice also suffered rectal prolapse.Conclusions Over-expression of IL-5 leads to dramatic increase of both total number and the proportion of eosinophils in whole white blood cells.The eosinophilic infiltration causes damages in the lung,liver,spleen and other organs.This mouse model is an excellent model for the study of eosinophilia.

关 键 词：嗜酸性粒细胞增多 基因工程小鼠 IL-5基因过表达 病理变化 

分 类 号：R557.5[医药卫生—血液循环系统疾病] R392.12[医药卫生—内科学]