CD3和CD20阳性细胞在人胎腭扁桃体的发育  

Development of CD3-positive cells and CD20-positive cells in human fetal palatine tonsil

作　　者：胡蓉[1] 苏敏[1] 黄悦[1] 李红[1] 许庭良[1] 姜俸蓉[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵阳550004

出　　处：《解剖学报》2011年第1期110-113,共4页Acta Anatomica Sinica

基　　金：贵州省教育厅基金资助项目(2001116)

摘　　要：目的探讨T、B细胞在人胎儿腭扁桃体内的发育情况。方法收集9～20周因故终止妊娠胎儿腭扁桃体22例,采用免疫组织化学方法,以细胞表面分化群(CD)抗体(CD3和CD20)染色分别显示T细胞和B细胞,用BioMiaspro图像分析软件对免疫反应阳性细胞进行计数,有关数据做统计学分析。结果妊娠9周时,胎儿腭扁桃体原基内已可见少量CD20阳性细胞和CD3阳性细胞,分布于黏膜上皮下方的间充质内。14周时,CD20阳性细胞数量显著增多,部分聚集于初级淋巴小结,其他的散在分布于弥散淋巴组织和隐窝上皮内;此时CD3阳性细胞仍较少,但隐窝上皮出现少量CD3阳性细胞浸润。17周时,初级淋巴小结增多。20周时,CD3阳性细胞显著增多,在固有层淋巴组织内散在分布或聚集成群。随着胎龄增加,两种阳性细胞逐渐增多(P<0.05或P<0.01),但CD20阳性细胞占多数(P<0.05)。结论胎儿腭扁桃体内出现T细胞和B细胞迁入的最早时间是第9周;胎儿腭扁桃体内初级淋巴小结的形成发生于第14周。Objective To study the development of T and B lymphocytes in human fetal palatine tonsil. Methods Totally 22 samples of human fetal palatine tonsil from the 9th to 20th gestational week （GW） were collected. T and B lymphocytes were displayed with cluster of differentiation （ CD ） , anti-CD3 antibody and anti-CD20 antibody by immunohistochemical ultrasensitive-SP method. The immunoreactive positive-cells were counted with BioMiaspro photographs analysis software and analysised with statistics software. Results In the 9th GW, there were rare CD3-positive cells and CD20-positive cells, distributed in the mesenchyme under the mucous membrane epithelium. In the 14th GW, the amounts of CD20-positive cells were significantly increasing, part of them were converged in primary lymphoid nodule, others were located in the diffused lymphoid tissue and crypt epithelium. At the same time, the amounts of CD3-positive cells were still to remain less, but a few of them were also infiltrated into the crypt epithelium. In the 17th GW, the number of primary lymphoid nodule was increaseing. In the 20th GW, the quantities of CD3-positive cells were significantly increasing, which were diffused distribution and formed grouping in the lymphoid tissue of lamina propria. The two types of positive-cells were increasing gradually with fetal age growing（ P 〈 0. 05 or P 〈 0.01 ） , but the numbers of CD20-positive cells were majority （P 〈 0. 05）. Conclusion In the 9th GW, T and B cells have emerged in the human fetal palatine tonsil; In the 14th GW, B cells converged and participated in forming primary lymphoid nodule.

关 键 词：T细胞 B细胞 CD3 CD20 腭扁桃体 免疫组织化学 人胎 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]