恩氟烷镇痛作用与脊髓5-HT_(1A)受体的关系  

Relationship between the analgesic effect of enflurane and the 5-HT1A receptors in the spinal cord of mice

作　　者：王芬[1] 严姝姝[1] 刘晶晶[1] 田艳艳[1] 宋歌[1] 杨晓琳[1] 陈默[1] 邢甜[1] 马涛[2] 袁力勇[3] 

机构地区：[1]徐州医学院麻醉学院,221002 [2]徐州医学院麻醉药理学教研室 [3]宁波市妇女儿童医院麻醉科

出　　处：《江苏医药》2011年第1期32-35,共4页Jiangsu Medical Journal

基　　金：宁波市自然科学基金资助项目(No2009A610140);江苏省高校自然科学基础研究资助项目(No09KJB320019)

摘　　要：目的探讨恩氟烷镇痛作用与脊髓5-羟色胺受体1A(5-HT1AR)之间的关系。方法腹腔注射恩氟烷建立镇痛模型,用甩尾法、热板法和扭体法分别观察鞘内注射5-HT1AR特异性拮抗剂p-MPPF对小鼠甩尾潜伏期(TFL)、热板法痛阈(HPPT)和扭体次数(WTs)的影响。结果腹腔注射恩氟烷可产生镇痛作用(P<0.05);单用p-MPPF 4μg/只或8μg/只对小鼠TFL、HPPT和WTs均无明显影响(P>0.05);两个剂量的p-MPPF均能使恩氟烷镇痛小鼠的TFL、HPPT缩短和WTs增加(P<0.05)。结论恩氟烷镇痛作用与脊髓5-HT1AR密切相关。Objective To investigate the relationship between the analgesic effect of enflurane and 5-HT1A receptor in the spinal cord of mice.Methods After the analgesic mice models were established by intraperitoneal injection of enflurane,the influences of p-MPPF（a specific antagonist of 5-HT1A receptors） intrathecal injection on the changes of tail-flick latency（TFL） in hot water tail-flick test,pain threshold in hot-plate test（HPPT） in hot-plate test and the writhing times（WTs） in acetic-induced writhing test were recorded.Results Intraperitoneal injection of enflurane alone produced analgesic effect（P0.05）.p-MPPF（4μg or 8μg in one mice） alone had no impact on TFL,HPPT and WTs（P0.05） in mice.But the two doses of p-MPPF reduced TFL or HPPT and increased WTs（P0.05） in analgesic mice with enflurane.Conclusion The analgesic effects of enflurane is related closely to 5-HT1A receptors in the spinal cord of mice.

关 键 词：5-羟色胺1A受体 p-MPPF 恩氟烷 

分 类 号：R614[医药卫生—麻醉学]