云南苗族Bardet-Biedl综合征家系外周血B淋巴母细胞系的建立  

Establishment of B lymphoblastoid cell lines of Miao pedigree with Bardet-Biedl syndrome

作　　者：沈涛[1] 寿涛[1] 林克勤[2] 易文[2] 华映坤[1] 董虹[1] 高建梅[1] 张进萍[1] 王林坪[1] 王金丽[1] 李丽[1] 严新民[1] 

机构地区：[1]云南省第一人民医院,昆明医学院附属昆华医院,昆明650032 [2]中国医学科学院医学生物学研究所

出　　处：《中华医学遗传学杂志》2011年第1期33-36,共4页Chinese Journal of Medical Genetics

基　　金：人力资源和社会保障部资助项目（[2009]416号）;云南省卫生厅科技计划项目（2009NS006）;云南省科技厅昆明医学院应用基础联合专项资金（2008CD041）

摘　　要：目的建立云南苗族巴德一毕氏综合征（Bardet-Biedlsyndrome，BBS）家系外周血B淋巴母细胞系，为研究BBS的发病机制、诊断和治疗提供长期的标本来源。方法取1个BBS核心家系成员新鲜肝素抗凝全血，采用EB病毒转化技术，并加入环孢霉素A（cyclosporineA，CyA）抑制T淋巴细胞，将B淋巴细胞转化成永生化淋巴母细胞系。结果成功建立了苗族BBS核心家系中12个个体的永生细胞株。结论EB病毒转化技术可有效保存原来个体完整的基因组，为以后的相关研究提供了长期的DNA资源。Objective To establish immortalized lymphoblastoid cell lines of a Miao core pedigree with Bardet-Biedl syndrome （BBS）, in order to provide a long-term source of material for research. Methods With Epstein-Barr virus transformation of B cells and addition of cyclosporine A to inhibit the activity of T cells, fresh anticoagulated blood samples with heparin were collected from 12 members of the core pedigree, and were used to establish the immortalized lymphoblastoid cell lines of B lymphocytes. Results Twelve immortalized lymphoblastoid cell lines of the core BBS pedigree were obtained successfully. Conclusion The immortalized B lymphoblastoid cell lines of the Miao pedigree with BBS can preserve the whole genome information and provide long-term research materials for BBS study.

关 键 词：巴德-毕氏综合征 家系 EB病毒 B淋巴细胞 永生化淋巴母细胞系 

分 类 号：R596[医药卫生—内科学]