天津地区汉族人群IL-10-592C／A基因多态性与冠状动脉支架术后再狭窄的关系  

Relationship of interleukin-10 gene polymorphism with restenosis after percutaneous coronary intervention in Chinese

作　　者：高静[1,2] 崔让庄[2] 刘寅[3] 毛用敏[2] 周津[3] 陈倩[2] 赵福梅[2] 杨桂铭[2] 刘婷[2] 

机构地区：[1]天津医科大学,300051 [2]天津市胸科医院心血管病研究所 [3]天津市胸科医院心内科

出　　处：《中华医学遗传学杂志》2011年第1期42-46,共5页Chinese Journal of Medical Genetics

基　　金：天津市卫生局课题资助项目（05KYZ90）

摘　　要：目的探讨中国天津地区汉族人群白细胞介素-10（interleukin-10，IL-10）-592C／A基因多态性的功能性以及其对经皮冠状动脉介入治疗（percutaneous coronary intervention，PCI）术后再狭窄的发病，PCI术后血清IL-10水平的影响。方法对437例接受PCI并进行冠状动脉造影随访的患者，按冠状动脉造影结果分为再狭窄组（166例）和非再狭窄组（271例），应用聚合酶链反应-限制性片段长度多态性方法检测IL10-592位点基因型和等位基因频率的分布；用酶联免疫吸附试验法测定2组PCI术前及PCI术后24h血清IL-10浓度，并比较两组间和各基因型间IL-10水平。结果（1）IL-10-592C／A基因型和等位基因频率在再狭窄组和非再狭窄组之间差异无统计学意义（P均〉0．05）；（2）PCI术后24h血清IL-10水平再狭窄组显著低于非再狭窄组[（82．67±35．02）ng／L vs （95．08±32．26）ng／L，P〈0．05]；（3）IL-10592位点A等位基因携带者（AA＋AC基因型）术后24h血清IL-10水平明显低于非携带者（CC型）[（86．13±34．77）ng／L vs．（102．50±27．52）ng／L，P〈0．05]；（4）再狭窄组A等位基因携带者术后24h血清IL-10水平明显低于非携带者[（78．51±34．09）ng／L vs．（102．19±33．66）ng／L，P〈0．05]；（5）再狭窄危险的多因素Logistic回归分析显示：急性冠状动脉综合征、术前狭窄程度、靶病变长度与冠状动脉内支架再狭窄呈正相关（OR值分别为5．90、1．86、2．83），术后24h血清IL-10水平、参照血管直径、支架直径与冠状动脉内支架再狭窄呈负相关（OR值分别为0．99、0．70、0．46）。结论（1）IL-10基因-592C／A多态性与中国天津地区汉族人群再狭窄发病无关；（2）IL-10是PCI术后早期的炎症细胞因子，术后24h血清IL-10水平为再狭窄的独立预测因素，携带A等位基因的个体可能通过降低其表型血清IL-10水平而增加了冠状�Objective To investigate the relationship of interleukin-10 gene （IL-10）polymorphism and the serum IL-10 level with restenosis after percutaneous coronary intervention （PCI） in Tianjin Chinese Han population and study the effect of IL-10 gene polymorphism on serum IL-10 level. Methods Four hundred and thirty-seven patients who successfully underwent PCI with a follow up angiography were divided into a restenosis group （n= 166） and non-restenosis group （n= 271）. The IL-10 gene promoter polymorphism at position -592 was determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Meanwhile their serum IL 10 level before and 24 h after PCI was determined by enzyme-linked immunosorbent assay （ELISA）. Results （1） There was no significant difference in frequencies of -592 genotypes and alleles between the two groups （P〉0.05） ;（2） The 24 h post-PCI IL-10 serum level of restenosis group was significantly lower than that of the non-restenosis group [（82.67±35.02） ng/L vs. （95.08±32.26） ng/L, P〈O. 05]; （3） The serum level of the A allele carriers （AA＋AC） was significant lower than that of the CC carriers [（86.13±34. 77） ng/L vs. （102.50±27.52） ng/L,P〈0.05]; （4） In the restenosis group, the 24 h post-PCI serum level of IL-10 in the A allele carriers was also significantly lower than that in those without the A allele E（78. 514±34.09） ng/L vs. （102. 19± 33.66） ng/L, P〈0. 05]; （5） Logistic regression analysis revealed positive correlations between acute coronary syndrome patients, pre PCI degree of stenosis, length of target stenosis lesion and restenosis （OR=5.90, 1.86, 2.83 respectively）; and there were negative correlations between 24 h post-PCI serum level of IL-10, the stent diameter, the diameter of reference vessel before stent implantation and restenosis （OR = 0.99, 0. 70, 0.46 respectively）. Conclusion （1） The IL 10 gene - 592 C/A polymorphism was not associated with restenosi

关 键 词：支架内再狭窄 白细胞介素-10 遗传多态性 

分 类 号：R541.4[医药卫生—心血管疾病]