卵母细胞线粒体DNA(mtDNA)拷贝数在卵巢储备功能下降中的研究  

Study on Mitochondrial DNA Content in Unfertilized Oocytes from Diminished Ovarian Reserve Patients

作　　者：赵红翠[1,2] 李艳萍[1] 

机构地区：[1]中南大学湘雅医院妇产科生殖医学中心,长沙410008 [2]解放军306医院生殖医学中心,北京100101

出　　处：《生殖与避孕》2011年第1期53-57,共5页Reproduction and Contraception

基　　金：湖南省科厅科技计划一般项目(No.05FJ4150)

摘　　要：目的:初步探讨卵母细胞线粒体DNA(mtDNA)拷贝数对卵巢储备功能下降患者生育潜能的影响。方法:选取IVF-ET治疗的不孕症患者80例,按照卵巢储备功能状态分为卵巢储备功能下降(DOR)组和卵巢储备功能正常(NOR)组,胚胎移植日收集患者自愿捐赠的未受精的废弃MII期卵母细胞,Real-timePCR方法定量检测比较卵母细胞mtDNA拷贝数。结果:共收集到废弃的MII期卵母细胞110个,其中DOR组46个,NOR组64个。DOR组卵母细胞mtDNA拷贝数下降(9.96×104±5.21×104vs1.77×105±7.27×104),组间具有显著性差异(P<0.05)。结论:卵巢储备功能下降患者卵母细胞mtDNA拷贝数减少,可能与其卵母细胞发育潜能下降相关。Objective：To explore the effect of mtDNA content on diminished ovarian reserve patients oocytes reproductive potential.Methods：Eighty infertility patients were enrolled.According to the ovarian reserve,they were divided into diminished ovarian reserve（DOR） group and normal ovarian reserve（NOR） group.Unfertilized abandonable MII oocytes were collected on embryo-transfer day.mtDNA content was quantified by real-time PCR.The two groups mtDNA was compared.Results：A total of 110 voluntarily donated unfertilized abandonable MII oocytes were collected,46 in DOR group,and 64 in NOR group.DOR group showed significantly lower mtDNA content（9.96×104±5.21×104 vs 1.77×105±7.27×104,P0.05）.Conclusion：The low mtDNA content in DOR patients oocyte may be related to their decreased oocyte developmental potential.

关 键 词：卵母细胞 卵巢储备功能 mtDNA拷贝数 实时荧光定量PCR 

分 类 号：R714.8[医药卫生—妇产科学]