B细胞活化因子及其受体BR3在强直性脊柱炎患者外周血白细胞中的表达及临床意义初探  

Expression of B-cell activation factor and its receptor BR3 in peripheral blood leukocytes from patients with ankylosing spondylitis and its clinical significance

作　　者：吴洪坤[1] 张玲珍[1] 周琳[1] 仲人前[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科全军临床免疫中心,上海200003

出　　处：《现代免疫学》2011年第1期17-21,共5页Current Immunology

基　　金：国家自然科学基金资助项目(30901368);上海市青年科技启明明星计划资助项目(09QA1407400)

摘　　要：为了探讨强直性脊柱炎(AS)患者外周血白细胞中B细胞活化因子(BAFF)及其受体(BR3)的表达水平及其临床意义。采用流式细胞术检测24例AS患者、30名健康体检者外周血白细胞上BAFF、BR3的表达水平,并比较其与红细胞沉降率(ESR)、C反应蛋白(CRP)、免疫球蛋白(IgG、IgA、IgM)的相关性。结果显示,AS患者中BAFF在单核细胞上的表达水平、BR3在淋巴细胞上的表达水平明显高于正常对照组(P<0.01),两者之间明显正相关(r=0.588,P=0.003)。同时与健康人相比,AS患者的ESR、血清IgG、IgA、CRP的含量明显升高(P<0.05);AS患者的外周血单核细胞上BAFF的表达水平分别与血清IgG、IgA、CRP、ESR含量之间呈正相关(r=0.813,P=0.000;r=0.665,P=0.000;r=0.514,P=0.010;r=0.417,P=0.043)、外周血淋巴细胞上BR3的表达水平也分别与血清CRP、ESR水平之间明显正相关(r=0.672,P=0.000;r=0.416,P=0.043)。研究表明,AS患者外周血白细胞中BAFF及其受体BR3的表达水平异常增多,提示BAFF及其受体可能与AS的发病及疾病进程有关。To investigate the expression of B-cell activation factor （BAFF） and its receptor BR3 in the peripheral blood of patients with ankylosing spondylitis （AS）, and to evaluate its clinical significance, the expression of BAFF and BR3 on the peripheral blood mononuclear cells were assayed with flow cytometry, and the correlation between their membrane-bound expression levels and the concentration of ESR, CRP, IgG, IgA and IgM was analyzed. The results showed that the expression of BAFF on peripheral blood monoeytes of AS patients was significantly higher than that of the healthy controls （P〈0.01）, closely paralleling with the expression of BR3 on peripheral blood lymphocytes of AS patients （r=0. 588, P= 0. 003）. The expression of BAFF and BR3 on the mononuclear cells in AS patients were both correlated with the level of IgG, IgA, CRP, and ESR （all P〈0.04）. These data suggest that the abnormal expressions of BAFF and its receptor BR3 may be involved in the pathogenesis of ankylosing spondylitis.

关 键 词：B细胞活化因子 受体 强直性脊柱炎 流式细胞术 

分 类 号：R392[医药卫生—免疫学]