β淀粉样蛋白诱导小胶质细胞表达Ⅰ-TAC  

The expression of IFN-inducible T cell alpha chemoattractant on microglia was induced by Beta-Amyloid

作　　者：胡海霞[1,2] 张秋玉[3] 吴林青[3] 苏东辉[3] 

机构地区：[1]福建中医药大学 [2]福建中西医结合研究院 [3]福建医科大学基础医学院免疫学系,福州350108

出　　处：《现代免疫学》2011年第1期22-27,共6页Current Immunology

基　　金：福建省教育厅重点项目(JA02217);福建医大博士启动基金(2009BS003)

摘　　要：已知小胶质细胞在中枢神经系统慢性炎症发展中起重要作用。本研究用β淀粉样蛋白等刺激小鼠小胶质细胞,RT-PCR检测Ⅰ-TAC的表达;用重组I-TAC刺激小鼠T细胞,流式检测黏附分子CD11a的表达,ELISA测定T细胞分泌细胞因子。结果表明,β淀粉样蛋白、LPS及IFN-γ均能诱导小胶质细胞表达Ⅰ-TAC;重组I-TAC不仅上调T细胞表达CD11a,还能促进T细胞分泌Th1型细胞因子IFN-γ。研究结果提示:β淀粉样蛋白诱导小胶质细胞表达Ⅰ-TAC,Ⅰ-TAC继而通过趋化T细胞抵达炎症局部,并调控T细胞表达黏附分子及向Th1方向极化,增进中枢神经系统慢性炎症反应的发展过程。It is well known that microglia play an important role in the pathogenesis of chronic inflammation of central nervous system. The aim of the present study was to detect the expression of chemokine IFN-inducible T cell alpha chemoattractant （I-TAC） on microglia and the potential role of I-TAC on T cell proliferation and differentiation. The expression of I-TAC mRNA was analyzed by RT-PCR, and the primary mouse T cells were isolated and purified by nylon wool. Effect of I-TAC on the proliferation of T cells was investigated by MTT assay, and the expression of CD11a on T cells was measured by FACS. It was found that the expression of I-TAC in microglia was induced by Beta-Amyloid, LPS and IFN-γ, and the recombinant murine I-TAC could promote the proliferation of T cells as well as induced T cell to produce IFN-γ. Furthermore, I-TAC significantly promoted the expression of CD11a （LAF-1） on T cells. The results of present study suggest that I-TAC play an important role in the development of chronic inflammation of neurological system.

关 键 词：趋化因子 I-TAC 小胶质细胞 T细胞 神经系统退行性变 

分 类 号：R392[医药卫生—免疫学]