香烟提取物对人支气管上皮细胞表达β-防御素2的作用研究  

Effect of Cigarette Smoke Extract on β-defensin 2 Expression by Human Bronchial Epithelial Cell(16HBE)

作　　者：刘蔺[1] 王开绿[1] 黄玲[1] 李文军[1] 郑晓凤[1] 刘艳群[1] 蒙琴[1] 

机构地区：[1]绵阳市第三人民医院呼吸科,621000

出　　处：《中国实用医药》2011年第1期30-31,共2页China Practical Medicine

摘　　要：目的 探讨香烟提取物(CSE)对人支气管上皮细胞表达人β防御素2(hBD2)的影响。方法利用不同浓度(4%和8%)的CSE刺激人支气管上皮细胞(16HBE),分别于0、6、12、24、48 h收集细胞总蛋白,运用酶联免疫吸附法(ELISA)测定hBD2的蛋白表达水平。结果 4%和8%CSE均诱导16HBE于6、12、24、48 h进行性hBD2表达下降。8%CSE干预组变化幅度大于4%CSE干预组。结论 CSE抑制人支气管上皮细胞表达hBD2,此机制可能参与香烟对气道防御的抑制作用。Objective To investigate the effect of cigarette smoke extract（CSE）on human β-defensin 2（hBD2）expression by human bronchial epithelial cell（16HBE）.Methods 16HBE cells were incubated with two different concentrations of CSE（4% and 8%）,and the cell protein was harvested at the experimental timepoints of 0 h,6 h,12 h,24 h,48 h,respectively.ELISA was used to assess the hBD2 protein expression level.Results After CSE（4% and 8%）stimulation,the hBD2 expression level significantly decreased at 6 h,12 h,24 h,48 h timepoints time-dependently,compared with the control.The expression of hBD2 in CSE 8% group had a larger change extent than CSE 4% group.Conclusion CSE suppresses hBD2 expression by 16HBE cells,and this mechanism may contribute to the inhibition of airway defense by cigarette smoke.

关 键 词：β防御素2 香烟提取物 支气管上皮细胞 

分 类 号：R563.9[医药卫生—呼吸系统]