小梅山猪Ghrelin基因的克隆及其在生殖轴的表达  

Cloning and Expression of Ghrelin Gene in Xiao Meishan Pig's Reproduction Axis

作　　者：张舒[1] 潘孝青[2] 丁家桐[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省农业科学院,南京210000

出　　处：《生物技术通报》2011年第2期110-114,125,共6页Biotechnology Bulletin

基　　金：江苏省自然科学基金项目(BK200505)

摘　　要：以小梅山母猪为试验材料,通过RT-PCR、克隆及荧光定量,研究Ghrelin在其生长发育不同阶段生殖轴表达情况,旨在探究Ghrelin对动物繁殖性能的影响。结果表明,初生、初情期至性成熟卵巢Ghrelin mRNA表达量呈上升趋势,不同阶段间差异显著(P<0.05)。性成熟时生殖轴不同组织Ghrelin mRNA表达量,卵巢明显高于垂体和下丘脑(P<0.05),而垂体与下丘脑间差异则不显著(P>0.05)。In order to research the biological functions of Ghrelin,its expression level during different growth development stage of Xiao Meishan pig＇s reproduction axis were analyzed.The results showed that the expression of Ghrelin mRNA expression level showed a upward trend and have a significant difference（P0.05）.The highest expression of Ghrelin mRNA was in sexual maturity pig＇s ovary which is sinificant higher than pituitary and hypothalamus（P0.05）,but the difference of expression level between pituitary and hypothalamus was not significant（P0.05）.

关 键 词：小梅山猪 GHRELIN 生殖轴 克隆 实时荧光定量PCR 

分 类 号：Q78[生物学—分子生物学]