需氧与厌氧配对培养在提高血培养阳性率中的优势  

Advantages of aerobic and anaerobic paired culture on raising positive rate of blood culture

作　　者：蒋伟燕[1] 李方去[1] 杨锦红[1] 王大选[1] 刘彩霞[1] 

机构地区：[1]温州医学院附属第二医院检验科,浙江温州325027

出　　处：《中华医院感染学杂志》2011年第3期622-623,共2页Chinese Journal of Nosocomiology

摘　　要：目的探讨同时做需氧与厌氧配对培养在提高血培养阳性率中的优势。方法在每例患者的不同部位同时抽取两份血液标本,一份注入需氧血培养瓶,一份注入厌氧血培养瓶,两者分别采用Bact/Alert 3D培养仪和BACTEC 9120培养仪进行培养。结果在3605份血培养中阳性共347份,阳性率为9.62%,其中仅需氧血培养阳性120份,占34.58%,仅厌氧血培养阳性88份,占25.36%,需氧与厌氧血培养均阳性139份,占40.06%;在88份仅厌氧血培养阳性中检出厌氧菌3份,占总阳性份数的0.86%。结论同时做需氧厌氧配对培养能够提高血培养的阳性率,常规开展厌氧血培养十分必要。OBJECTIVE To discuss the advantage of aerobic and anaerobic paired culture on raising the positive rate of blood culture.METHODS Two samples were collected from each patient＇s different sites at the same time,one was injected into an aerobic blood culture bottle,and the other was injected into an anaerobic blood culture bottle.The aerobic and anaerobic blood culture bottles were cultured by using Bact/Alert 3D cultivator and BACTEC 9120 cultivator respectively.RESULTS There were 347 isolates（9.62%） recovered from 3605 paired aerobic and anaerobic blood culture bottles,120 positive isolates（34.58%） recovered only from aerobic bottles,and 88 isolates（25.36%） recovered only from anaerobic bottles,139 isolates（40.06%） recovered from both of the two bottles.3 cases of anaerobic bacteria were recovered in 88 isolates,accounting for 0.86%.CONCLUSION Using paired aerobic and anaerobic blood culture bottles can raise the positive rate of blood culture.It is necessary to carry out anaerobic blood culture generally..

关 键 词：血流感染 需氧培养 厌氧培养 厌氧菌 血培养 

分 类 号：R378[医药卫生—病原生物学]