CD4^+CD25^+调节性T细胞对人外周血内皮祖细胞增殖、迁移、黏附的影响  

The effect of CD4^+CD25^+ regulatory T cells on the proliferation,migration and adhesion of endothelial progenitor cells

作　　者：谢培益[1] 苏又苏[1] 汤海燕 方红成[1] 何少林[3] 李大主[3] 

机构地区：[1]广东医学院附属深圳南山人民医院心内科,广东深圳518052 [2]深圳市职业病防治院检验科 [3]华中科技大学同济医学院协和医院心内科

出　　处：《临床心血管病杂志》2011年第1期63-66,共4页Journal of Clinical Cardiology

摘　　要：目的:研究CD4+CD25+调节性T细胞对体外培养的人外周血内皮祖细胞(EPCs)增殖、迁移、黏附的影响。方法:密度梯度离心法分离培养人外周血单个核细胞,经FITC-UEA-I和Dil-acLDL双染色鉴定为正在分化的EPCs。进一步采用流式细胞仪检测其表面标志CD34、CD133。磁性细胞分离器(MACS)分离CD4+CD25+调节性T细胞及CD4+CD25-T细胞。将EPCs分别与CD4+CD25+调节性T细胞或CD4+CD25-T细胞共培养36h。采用MTT比色法、transwell小室、细胞计数法观察EPCs增殖、迁移、黏附能力。结果:与对照组和CD4+CD25-T细胞相比,与CD4+CD25+调节性T细胞共培养EPCs增殖、迁移、黏附能力显著增强,且呈浓度依赖性增强。结论:CD4+CD25+调节性T细胞可显著促进EPCs增殖、迁移、黏附能力,此作用可能是其抗AS作用机制之一。Objective：To investigat the effect of CD4＋CD25＋ regulatory T cells（Tregs） on the proliferation,migration and adhesion of endothelial progenitor cells（EPCs） from human circulating blood in vitro.Method：Tregs were isolated from lymphocyte suspensions by magnetic cell sorting column and analyzed by flow cytometry.Human blood mononuclear cells were isolated with Ficoll by density gradient centrifugation.EGM-2MV culture fluid was added,and then cells were plated on dishes coated with human fibronectin.After 7 days,the cells were identified with immunofluorescence and flow cytometry.The cells were cultured alone（control groups）,with CD4＋CD25-T cells（CD25-groups）,or CD4＋CD25＋Tregs（Tregs groups） for 36 hours.The proliferation,migration and adhesion activities of EPCs were determined with MTT assay,transwell assay and adhesive assay,respectively.Result：Compared with control groups and CD25--groups,the proliferative,migratory and adhesive activities of EPCs were significantly enhanced after treated with CD4＋CD25＋Tregs（P0.05 and P0.01）.Moreover,the proliferative,migratory and adhesive activities of EPCs were increased dose-dependently.Conclusion：Results collectively suggest that CD4＋CD25＋Treg cells may improve the proliferative,migratory and adhesive activities of EPCs.

关 键 词：动脉粥样硬化 调节性T细胞 内皮祖细胞 增殖 迁移 黏附 

分 类 号：R543.5[医药卫生—心血管疾病]