环指蛋白RING1与A型核纤层蛋白的细胞内相互作用  

In vivo Interation of RING1 and Lamin A

作　　者：陈维春[1,2] 宋杰[1] 刘振杰[2,3] 蒋智文[1,2] 袁源[1] 郑慧玲[1,2] 刘新光[1,2] 

机构地区：[1]广东医学院衰老研究所,东莞523808 [2]广东医学院生物化学与分子生物学研究所,湛江524023 [3]广东省中医院枪验科,广州510120

出　　处：《生物化学与生物物理进展》2011年第1期55-59,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(30672205,30871440,30900739);广东省自然科学基金(7301506,9252402301000002)资助项目;广东省高校自然科学研究重点项目(06Z015);东莞市科技计划项目(2008108101062);湛江市科技攻关项目(2009C3104005)~~

摘　　要：环指蛋白RING1能结合DNA并抑制基因的转录.采用酵母双杂交方法从人骨骼肌文库中筛选出了与A型核纤层蛋白(lamin A)结合的RING1蛋白,回复杂交酵母能在缺陷培养基上生长.RING1与绿色荧光蛋白融合载体转染HEK293细胞,激光共聚焦显微观察发现RING1能与带红色荧光蛋白的lamin A蛋白在细胞核周围共定位.免疫共沉淀结果证明RING1与lamin A能够相互作用.结果证明了一个新的lamin A结合蛋白,为揭示lamin A影响基因表达乃至细胞衰老提供了依据.RING1 can bind to DNA and inhibit some gene transcription.RING1 was identified to interact with lamin A by screening a human skeletal muscle library in a yeast two-hybrid interacting screen.The results of one to one reverse hybridization indicated that AH109 transfected with RING1 and lamin A constructs could grow on SD/-Leu/-Trp/-Ade/-His medium.RING1 was inserted into pEGFP-N1 vector and the constructs including pDsRed-LA and pEGFP-RING1 was used to transfect HEK293 cells.Visualized by laser confocal microscopy,RING1 and Lamin A were co-located around nucleus.Interatcion of RING1 and lamin A was comfirmed by immunoprecipitations.These results showed a new lamin A binding protein that can present some evidence for lamin A acting on gene expression and cell senescence.

关 键 词：RING1 核纤层蛋白A 相互作用 酵母双杂交 

分 类 号：Q51[生物学—生物化学]