加味虎杖散对自身免疫性前列腺炎模型大鼠炎症因子MCP-1及PDGF-BB表达的影响  

Effects of Jiawei Huzhang San Decoction on the expressions of inflammatory factors MCP-1 and PDGF-BB in rat models of experimental autoimmune prostatitis

作　　者：周青[1] 田雪飞[2] 龚秀英[2] 冯亚兰[2] 胡华[1] 

机构地区：[1]湖南中医药大学第一附属医院外科,湖南长沙410007 [2]湖南中医药大学中西医结合学院,湖南长沙410208

出　　处：《中华男科学杂志》2011年第1期83-88,共6页National Journal of Andrology

基　　金：湖南省自然科学基金项目(10JJ3004)~~

摘　　要：目的:观察加味虎杖散对自身免疫性前列腺炎模型大鼠炎症因子单核细胞趋化蛋白1(MCP-1)、血小板源生长因子BB(PDGF-BB)基因及蛋白表达的影响。方法:将72只健康雄性Wistar大鼠随机分为6组,每组12只,除正常组外,其余5组应用大鼠前列腺蛋白提纯液辅以免疫佐剂制备实验性自身免疫性前列腺炎大鼠模型,造模60d时正常组、模型组予生理盐水,西药组予消炎痛,加味虎杖散大剂量、中剂量、小剂量组分别按生药量0.445、0.223、0.089g/kg体重予中药复方连续灌胃,各组给药30d时处死,采用免疫组化、荧光定量RT-PCR等方法检测炎症因子mRNA及蛋白的表达。结果:加味虎杖散大、中、小剂量组前列腺组织MCP-1、PDGF-BBmRNA表达水平(MCP-1:0.31±0.14、0.49±0.21、0.62±0.28;PDGF-BB:0.50±0.22、0.54±0.17、0.71±0.29)及蛋白的IA值(MCP-1:677±208、725±311、1302±884;PDGF-BB:1265±698、1347±827、1655±812)与模型组(MCP-1mRNA:1.12±0.43;MCP-1蛋白:2201±934;PDGF-BBmRNA:1.14±0.51;PDGF-BB蛋白:2754±852)比较显著降低(P<0.05),且大、中剂量组作用要优于小剂量组(P<0.05);西药组MCP-1(mRNA:0.71±0.34;蛋白:1824±1157)、PDGF-BB(mRNA:1.08±0.37;蛋白:2493±924)表达水平显著高于加味虎杖散各组(P<0.01)。结论:调控炎症因子MCP-1、PDGF-BB可能是加味虎杖散治疗慢性非细菌性前列腺炎的重要分子机制之一。Objective：To study the effects of Jiawei Huzhang San（JWHZS） decoction on the expressions of the inflammatory factors monocyte chemoattractant protein-1（MCP-1） and platelet-derived growth factor-BB（PDGF-BB） on experimental autoimmune prostatitis in rats.Methods：Twelve male Wistar rats were taken as normal controls,and models of experimental autoimmune prostatitis were established in another 60 by injection of SC purified prostate protein with FCA,and then divided into five groups to be treated with normal saline,indomethacin,high-dose JWHZS（0.445 g/kg）,medium-dose JWHZS（0.223 g/kg） and low-dose JWHZS（0.089 g/kg）,respectively.All the rats were sacrificed at 30 days after the treatment for detection of the mRNA and protein expressions of inflammatory factors by immunohistochemistry and fluorescent quantitative RT-PCR.Results：In the high-,medium-and low-dose JWHZS groups,the mRNA expressions of MCP-1（0.31±0.14,0.49±0.21 and 0.62±0.28） and PDGF-BB（0.50±0.22,0.54±0.17 and 0.71±0.29）,and the protein expressions of MCP-1（677±208,725±311 and 1 302±884） and PDGF-BB（1 265±698,1 347±827 and 1 655±812） were significantly lower than in the model control group（MCP-1 mRNA：1.12±0.43;MCP-1 protein：2 201±934;PDGF-BB mRNA：1.14±0.51;PDGF-BB protein：2 754±852）（P0.05）.And JWHZS exhibited a significantly better activity at high and medium doses than at a low dose（P0.05）.In the indomethacin control group,both the mRNA and protein expressions of MCP-1（0.71±0.34 and 1 824±1 157） and PDGF-BB（1.08±0.37 and 2493±924） were markedly higher than in the JWHZS groups（P0.01）.Conclusion：Down-regulation of the inflammatory factors MCP-1 and PDGF-BB may be the important molecular mechanism of JWHZS acting on experimental autoimmune prostatitis.

关 键 词：加味虎杖散 慢性非细菌性前列腺炎 自身免疫性前列腺炎 炎症因子 单核细胞趋化蛋白1 血小板源生长因子BB 模型大鼠 

分 类 号：R285.5[医药卫生—中药学]