盐度胁迫对鲻鱼幼鱼鳃丝Na^+/K^+-ATP酶活力和体含水量的影响  

Effects of Salinity Stresses on Gill Na^+/K^+-ATPase(NAK)Activity and Body Moisture in Juvenile Grey Mullet Mugil cephalus

作　　者：于娜[1,2] 李加儿[1] 区又君[1] 范春燕[1,2] 张建生 

机构地区：[1]中国水产科学研究院南海水产研究所,广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]省级饶平石鲈科鱼类良种场,广东饶平515723

出　　处：《动物学杂志》2011年第1期93-99,共7页Chinese Journal of Zoology

基　　金：广东省科技计划项目(No.2007B020708005)

摘　　要：研究了环境盐度急性胁迫对鲻鱼幼鱼(Mugil cephalus)鳃丝Na+/K+-ATPase(NKA)活性及体内含水量的影响。结果表明,将幼鱼从S33(盐度33)的对照组中直接转移至S0(盐度0)、S10(盐度10)、S20(盐度20)、S33和S40(盐度40)的水体中,随盐度降低各盐度处理的死亡率迅速升高。各处理组鱼的鳃丝NKA活性在最初3 d内出现一定波动,随后变化平稳。实验结束时(12 d),NKA活性随盐度梯度呈"∩"型分布,最终(12 d时),S10、S20处理组酶活性显著高于其他处理组(P<0.05),S33处理组活性最低。不同盐度的去内脏组和全鱼组含水量比较结果均为S0>S20>S10>S33>S40。从总体结果来看,鲻鱼幼鱼在9 d左右适应不同盐度的水环境。在稳定期,全鱼组(12 d)与去内脏组(14 d)比较,除S0处理组的鲻鱼含水量去内脏组大于全鱼组,其余各组都是全鱼组大于去内脏组。In this paper,the gill Na ＋/K ＋ -ATPase （NKA） activity and the body moisture in juvenile grey mullet Mugil cephalus were investigated after 12 d stress at different ambient salinities （0 -40）. The results showed that the mortality rate of the juvenile grey mullet were 31.7% ,28.3% ,6.7% ,3.3% and 13.4% ,respectively when the fishes were suddenly transferred from control group（ salinity was 33） into SO （salinity was 0） , S10, S20, S33 （control） and S40 treatments. The gill NKA activity fluctuated in the first 3 d, and then changed smoothly in all treatments. At the end of experiment （12 d） ,the NKA activity varied with salinity grade in a ＂N ＂shape, being significantly higher in salinity Slo and S2o （ P 〈 0. 05 ） groups while the lowest in S33 group. The body moisture content of visceral removal group showed a trend of So 〉 S2o 〉 S^0 〉 S^3 〉 S40 while that of whole fish group showed a trend of So 〉 S2o 〉 Sl0 〉 S33 〉 S4o. All the results revealed that the juvenile grey mullet could adapt to different salinity environments in 9 days. Comparing with the visceral removal group （14 d）, the moisture content of whole fish group （ 12 d） was higher except for the SO treatment group.

关 键 词：盐度胁迫 鲻鱼 Na+/K+-ATP酶 体含水量 

分 类 号：S917.4[农业科学—水产科学]