褪黑素对大鼠实验性脊髓损伤的神经保护作用  

Neuroprotective effect of melatonin on experimental spinal cord injury in rats

作　　者：胡爱民[1] 张治国[1] 侯志贞[1] 曾益华[1] 许瑞佳[1] 尹延伟[1] 

机构地区：[1]空军总医院急诊部,北京100142

出　　处：《解放军医学杂志》2011年第2期164-166,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的观察不同剂量褪黑素对实验性脊髓损伤(SCI)大鼠早期脂质过氧化水平及超微结构的神经保护作用。方法采用钳夹法在大鼠T10水平建立SCI模型。60只SCI模型大鼠随机分为6组:模型Ⅰ组仅行椎板切除,模型Ⅱ组行椎板切除+钳夹,对照组损伤后立即给予5%无水乙醇0.2ml,甲泼尼龙治疗组给予甲泼尼龙30mg/kg,褪黑素治疗Ⅰ组给予褪黑素50mg/kg,褪黑素治疗Ⅱ组给予褪黑素100mg/kg。于伤后24h检测各组大鼠受损部位脊髓丙二醛(MDA)含量并进行超微结构病理改变评分。结果模型Ⅰ组及各治疗组MDA含量明显低于模型Ⅱ组和对照组(P<0.05),褪黑素治疗组与甲泼尼龙治疗组间差异无统计学意义(P>0.05),两个褪黑素治疗组之间差异亦无统计学意义(P>0.05)。超微组织病理改变评分结果显示,褪黑素治疗Ⅱ组的神经保护作用与甲泼尼龙治疗组相当(P>0.05),褪黑素治疗Ⅱ组的结果优于褪黑素治疗Ⅰ组(P<0.05)。结论褪黑素可抑制实验性脊髓损伤早期脂质过氧化水平,从而发挥神经保护作用。Objective To observe the neuroprotective effect of different doses of melatonin on early lipid peroxidation level and ultra-structure in experimental spinal cord injury（SCI） rats.Methods The SCI model of rat was established by an aneurysm clip at the level of T10.Sixty model rats were randomly divided into 6 groups（10 each）： model I group received only laminectomy;model II group received laminectomy and aneurysm clip;the SCI rats in control group were given 0.2ml of 5% ethanol immediately after injury;the SCI rats in methylprednisolone treatment group were given 30mg/kg of methylprednisolone;melatonin treatment I and II group received melatonin with dosage of 50 and 100 mg/kg,respectively.The malonaldehyde（MDA） level at the injured sites of the spinal cord at 24 hours after trauma was detected,and the score of ultrastructural histopathological changes was also made.Results The MDA levels in model I and all treatment groups were significantly lower than those in model II and control group（P〈0.05）.There were no statistical difference between melatonin and methylprednisolone treatment group（P〉0.05）,and the same was between the two melatonin treatment groups（P〉0.05）.The scoring of ultrastructural histopathological changes showed that melatonin treatment II group had the same neuroprotective effect as in methylprednisolone group（P〉0.05）,while the effect of melatonin treatment II group was significantly better than that of melatonin treatment I group（P〈0.05）.Conclusions Melatonin can inhibit the early lipid peroxidation of experimental spinal cord injury,and possess the neuroprotective effect on the ultra-structure.

关 键 词：脊髓损伤 褪黑激素 脂质过氧化作用 

分 类 号：R651.21[医药卫生—外科学]