原核表达Arresten蛋白抑制血管生成作用  

The Inhibitory Effect of Arresten Protein on Angiogesis

作　　者：吕懿[1] 郑金平[1] 

机构地区：[1]山西医科大学卫生毒理学教研室,太原030001

出　　处：《中国生物工程杂志》2011年第1期19-23,共5页China Biotechnology

基　　金：山西省科技攻关(042082);山西省高校产业化(20080017)资助项目

摘　　要：目的:研究原核表达的Arresten蛋白纯化品对血管内皮细胞及血管生成的抑制作用。方法:MTT法检测Arresten蛋白对人脐静脉内皮细胞(HUVEC)增殖的影响;流式细胞仪分析Arresten蛋白作用下HUVEC凋亡的情况;细胞迁移实验观察Arresten蛋白对HUVEC迁移能力的影响;鸡胚绒毛尿囊膜(CAM)实验观察Arresten蛋白对新生血管的抑制情况。结果:原核表达的Arresten蛋白纯化品能特异性地抑制HUVEC的增殖、迁移,诱导HUVEC的凋亡,并在一定范围内呈现出剂量—效应关系。Arresten蛋白能有效抑制鸡胚尿囊膜血管的生长(P<0.01)。结论:原核表达的Arresten蛋白纯化品对内皮细胞有特异的抑制作用,能有效抑制血管生成。Objective：To investigate the effect of Arresten protein on proliferation and migration of human umbilical vein endothelial cell（HUVEC） and the inhibitory effect of Arresten protein on angiogenesis.Methods：Arresten protein was incubated with HUVEC in vitro：The effect on proliferation of HUVEC was examined using MTT assay.The effect on apoptosis of HUVEC was monitored by FCM.The effect on migration of HUVEC was examined by Boyden Chamber.Chicken chorioallantoic membrane（CAM） angiogenesis model was used to identify the influence of Arresten protein on neovascularization.Results：Arresten protein inhibits the proliferation and migration of HUVEC and promotes its apoptosis effectively;And the effect was dose-dependent.Compared with PBS group,the vessels of Chicken chorioallantoic membrane（CAM） in Arresten protein treated groups were obviously damaged（P〈0.01）.So Arresten protein can significantly inhibit angiogenesis of CAM.Conclusion：Arresten protein inhibits angiogenesis through the inhibition on HUVEC.

关 键 词：ARRESTEN 血管抑制因子 原核表达 血管生成 

分 类 号：Q786[生物学—分子生物学]