rSj26-Sj32融合蛋白-IgG-ELISA用于急性日本血吸虫病的诊断价值  

The diagnostic value of rSj26-Sj32-IgG-ELISA for acute schistosomiasis japonica

作　　者：王敏[1] 李文桂[1] 蔡世飞[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,400016

出　　处：《中国地方病学杂志》2011年第1期39-43,共5页Chinese Jouranl of Endemiology

基　　金：重庆市科委地方病重大专项基金（2008AB5055、2008AB5008、2008AB5054）

摘　　要：目的 探讨rSj26-Sj32融合蛋白-IgG-ELISA用于急性日本血吸虫病的诊断价值.方法 利用纯化的rSj26-Sj32融合蛋白和日本血吸虫成虫粗抗原（SjAWA）建立IgG-ELISA方法检测急性日本血吸虫病患者血清,并以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎、肺结核患者和健康人血清作对照.结果 rSj26-Sj32和SjAWA检测急性日本血吸虫病患者的敏感性和特异性分别为90.00%（45/50）、97.67%（42/43）和92.00%（46/50）、97.67%（42/43）,二者比较差异无统计学意义（x2值均为0.0,P均＞0.05）;SjAWA与泡型棘球蚴病患者血清的交叉反应率为20.00%（2/10）,rSj26-Sj32与泡型棘球蚴病患者血清未见交叉反应,二者比较差异无统计学意义（x2=0.5,P＞0.05）;rSj26-Sj32和SjAWA与华支睾吸虫病和卫氏并殖吸虫病患者血清的交叉反应率分别为14.29%（3/21）、7.69%（1/13）和19.05%（4/21）、7.69%（1/13）,与囊型棘球蚴病、乙型肝炎和肺结核患者血清均未见交叉反应,二者比较差异无统计学意义（x2值均为0.0,P均＞0.05）.rSj26-Sj32-IgG-ELISA和SjAWA-IgG-ELISA诊断急性日本血吸虫病的阳性预告值、阴性预告值和诊断效率分别为97.83%（45/46）、89.36%（42/47）、93.55%（87/93）和97.87%（46/47）、91.30%（42/46）、94.62%（88/93）,二者比较差异无统计学意义（x2值均为0.0,P均＞0.05）.结论 rSj26-Sj32融合蛋白可用于急性日本血吸虫病的免疫诊断.Objective To study the diagnostic value of rSj26-Sj32-IgG-ELISA for acute schistosomiasis japonica. Methods Purified rSj26-Sj32 fusion protein and crude Schistosoma japonicum antigen （SjAWA）were used to establish IgG-ELISA to detect serum of patients with acute schistosomiasis, and clonorchiasis sinensis,paragonimiasis westermani, alveolar echinococcosis, cystic echinococcosis, type B hepatitis, lung tuberculosis patients and healthy human serum were used as control. Results The sensitivity and specialty were 90.00%（45/50） ,97.67% （42/43） and 92.00% （46/50）,97.67% （42/43） in detection of acute schistosomiasis japonica with rSj26-Sj32and SjAWA, respectively, and the difference was not statistically significant（x2 were both 0.0, all P 〉0.05）. The serum cross-reaction reactivity was 20.00%（2/10） in patients with alveolar echinococcosis with SjAWA,but no cross-reaction with rSj26-Sj32, the difference was not statistically significant（x2 = 0.5, P 〉 0.05）. The serum cross-reactivity were 14.29% （3/21 ）, 7.69% （1/13） and 19.05% （4/21 ）, 7.69% （1/13） among patients with clonorchiasis sinensis and paragonimiasis westermani by rSj26-Sj32 and SjAWA, but no cross reaction with type B hepatitis and lung tuberculosis patients, the difference was not statistically significant （x2 were both 0.0, all P 〉 0.05）. The positive predictive value, the negative predictive value and the diagnostic efficiency with acute schistosomiasis japonicum by rSj26-Sj32-IgG-ELISA and SjAWA-IgG-ELISA were 97.83% （45/46）,89.36% （42/47）,93.55% （87/93）and 97.87% （46/47）,91.30% （42/46）,94.62% （88/93）, respectively, and the difference was not statistically significant （x2 were both 0.0, all P 〉 0.05）. Conclusion rSj26-Sj32 fusion protein can be used for the immune diagnosis of acute schistosomiasis japonica.

关 键 词：血吸虫病 日本 诊断 酶联免疫吸附测定 免疫学试验 

分 类 号：R5[医药卫生—内科学]