强功率UVA1对兔耳增生性瘢痕模型影响的机制研究  

Mechanisms of influence of high-power UVA1 radiation on the formation of hypertrophic scar in rabbits

作　　者：张彤[1] 夏群力[2] 郑捷[2] 

机构地区：[1]上海瑞金医院集团闵行区中心医院皮肤科,201100 [2]上海交通大学医学院附属瑞金医院皮肤科

出　　处：《中华皮肤科杂志》2011年第1期39-43,共5页Chinese Journal of Dermatology

基　　金：上海市教育发展基金会产学研基金（2005）

摘　　要：目的 探讨不同剂量UVA1对全层皮肤缺损诱导的兔耳增生性瘢痕模型的影响的可能机制.方法 24只新西兰白兔双耳腹面手术切除2 cm×5 cm全层皮肤至筋膜建立兔耳增生性瘢痕模型后,随机分成4组,每组6只,将每只兔左耳分别于伤后即刻（U0）、1个月（U1）、2个月（U2）、3个月（U3）开始强功率UVA1照射,右耳为非照射组;各照射组分为两个剂量组[60 J/cm2（M）,110 J/cm2（H）],分别在照射前后进行MMP-1、TIMP-1、TGF-β1、PCNA和α-SMA的免疫组织化学染色和透射电镜检查.结果 与照射前比较,中高剂量组照射后瘢痕处MMP-1表达：U1组分别为10.43±1.61、11.16±1.57;U2组分别为8.63±2.61、7.33±1.58;U3组分别为5.74±1.43、3.11±0.27;均显著增加（P＜0.05）.TGF-β1表达：U1组分别为12.51±4.13、12.02±5.02;U2组分别为18.74±6.42、19.69±4.52;U3组分别为20.51±1.78、29.45±6.55.PCNA表达：U1组分别为2.67±0.44、2.04±0.65;U2组分别为4.50±0.97、5.82±0.68;U3组分别为7.45±1.47、8.16±1.07;均显著降低（P＜0.05）.只有高剂量组显著降低TIMP-1表达,U1组为12.74±4.58,U2组为15.17±3.26,U3组为20.72±3.31（P＜0.05）.只有U1H、U1M、U2H组α-SMA表达（1.33±0.34、2.04±0.20、3.60±1.75）显著降低（P＜0.05）.U0组：与对照组比较,高剂量组MMP-1表达（2.25±0.38）显著降低（P＜0.05）,而TGF-β1表达（23.90±2.92）显著增加（P＜0.05）.中、高剂量组PCNA（7.42±0.65、7.59±0.31）、TIMP-1（29.82±1.94、33.51±1.19）及α-SMA表达（6.31±0.61、2.97±0.56）均显著增加（P＜0.05）.透射电镜结果显示：强功率UVA1照射后胶原纤维直径变细;成纤维细胞胞质变少,细胞器欠发达,多数为静止的纤维细胞.结论 UVA1对瘢痕的作用可能与其抑制TGF-β1、TIMP-1、PCNA和α-SMA的表达,同时促进MMP-1的表达,从而促进基质蛋白降解及抑制成纤维细胞和肌成纤维细胞的增值活性有关.如果UVA1过早干预,则会出现相�Objective To study the possible mechanisms of influence of different doses of UVA1 on the development of hypertrophic scar in rabbit ears induced by excision of full-thickness skin. Methods A hypertrophic scar model was established by excision of full-thickness skin on the ventral surface of rabbit ears.A total of 24 New Zealand white rabbits were randomly and equally divided into 4 groups to receive UVA1 radiation on the left ear immediately （U0 group）, 1 month （U1 group）, 2 months （U2 group） and 3 months （U3 group） after the excision, respectively, and each group were classified into two subgroups to be irradiated with UVA1 of 60 （middle） and 110 （high） J/cm2, respectively, for 30 sessions. The right ears served as the control without irradiation. Skin samples were obtained from the ears of rabbits before the first and after the last irradiation, transmission electron microscopy （TEM） was used to observe the ultra-structure and morphology of collagen fiber and fibroblasts, and immunohistochemical staining was performed to measure the expressions of matrix metalloproteinases （MMP）-1, tissue inhibitor of metalloproteinase （TIMP）-1, transforming growth factor （TGF）-β1, proliferating cell nuclear antigen （PCNA） and α-smooth muscle actin （α-SMA） in skin samples. Results Compared with the unirradiated skin, irradiated skin showed higher expression levels of MMP-1 （P ＜ 0.05）, which were 10.43 ± 1.61 and 11.16 ± 1.57 in middle- and high-U1 group, 8.63 ± 2.61 and 7.33 ± 1.58 in middle- and high-U2 gorup, 5.74 ± 1.43 and 3.11 ± 0.27 in middle- and high-U3 group respectively. The expression level of TGF-β1 in irradiated skin was 12.51 ± 4.13 and 12.02 ± 5.02 in middle- and high-U1 group, respectively, 18.74 ± 6.42 and 19.69 ± 4.52 in middle- and high-U2 group, respectively, 20.51 ± 1.78 and 29.45 ± 6.55 in middle- and high-U3 group, respectively. A significant decrease was observed in the expression of PCNA in irradiated skin in middle- and high-U1 grou

关 键 词：瘢痕 模型 动物 紫外线 

分 类 号：R751[医药卫生—皮肤病学与性病学]