骨髓基质细胞对血管性痴呆大鼠的保护作用  

Protective effects of bone marrow stromal cells on aged rats with vascular dementia

作　　者：黄敬[1] 杨嘉君[2] 陈玉娟[1] 边伟红[1] 余静[1] 刘学原[1] 

机构地区：[1]上海市第十人民医院神经内科,200072 [2]上海市第六人民医院神经内科,200023

出　　处：《中华神经医学杂志》2011年第1期10-14,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（81000492）

摘　　要：目的 观察BMSCs移植对老化血管性痴呆大鼠模型认知功能的改善情况.方法 对皮下注射4周D-半乳糖（D-gal）的大鼠行双血管结扎术（2VO）,术后24 h移植BMSCs入侧脑室下区（SVZ）.6周后行水迷宫检测,比较各组大鼠认知功能改变情况,同时行免疫荧光组织化学染色,观察BMSCs向神经元转化情况.结果 D-gal加2VO大鼠在水迷宫中所行距离及所花时间均较对正常大鼠明显增加,差异有统计学意义（P＜0.05）,提示D-gal加2VO大鼠认知功能有明显缺损,符合老化血管性痴呆大鼠特征.移植6周后BMSCs围绕侧脑室周边分布,有1%的细胞分化为胶质细胞,2%的细胞分化为神经元.同时观察到移植BMSCs 6周后大鼠在水迷宫中所行距离及所花时间较老化血管性痴呆大鼠明显减少,差异有统计学意义（P＜0.05）,但泳动速度没有明显差异（P＞0.05）.结论 D-gal皮下注射加2VO模型能导致明显的认知功能缺损,而BMSCs移植能显著改善这种认知功能损害,考虑与BMSCs能促进宿主脑内神经功能重塑有关.Objective To evaluate whether bone marrow stromal cells （BMSCs） transplantation can improve the cognitive function of aged rats with vascular dementia. Methods Thirty rats were equally ramdomized into normal control group and 4 treatment groups; the 4 treatment groups received subcutaneous injection of D-galactose （D-gal） for 4 weeks; and then, two-vessel occlusion （2VO） was performed in 3 of the treatment groups; and 24 h after 2VO, D-gal＋2VO＋saline group and D-gal＋2VO＋BMSCs group were subjected to stereotactic injection of normal saline and BMSCs into the subventricular zone （SVZ）, respectively. The cognitive function was examined by Morris water maze test 6 weeks after stereotactic injection; immunofluorescence staining was employed to observe the transplantation ratio of BMSCs to neurons. Results Increased times and distances during Morris water maze in rats of the D-gal＋2VO group, D-gal＋2VO＋saline group and D-gal＋2VO＋BMSCs group were noted as compared with those in the controls, indicating that the cognitive function of rats in these 3 groups was obviously impaired; these rats had the characteristics of having vascular dementia.Transplanted BMSCs in the D-gal＋2VO＋BMSCs group distributed around the lateral ventricles, and acquired the phenotypes of neurons （2%） and astrocyte （1%） 6 weeks after the transplantation. In addition, compared with that in rats of the D-gal＋2VO group and D-gal＋2VO＋saline group, the cognitive dysfunction of rats in the D-gal＋2VO＋BMSCs group was improved （needing less time and swimming shorter distance, no difference in speed of swimming）. Conclusion The D-gal injection plus 2VO can result in cognitive dysfunction of rats, and the engrafted BMSCs may exhibit the beneficial effect on cognitive function.Neural function remolding caused by interaction between BMSCs and host brain may be responsible for the function improvement

关 键 词：血管性痴呆 老化 骨髓基质细胞 认知功能 

分 类 号：R285.5[医药卫生—中药学]