EGb761对急性大鼠脊髓损伤后血脊髓屏障的保护作用及其机制研究  

Protective effect of extract of Ginkgo biloba on blood-spinal cord barrier after acute spinal cord injury in rat model

作　　者：邵为[1] 花曼曼[2] 龚凯[3] 陈宇飞[1] 闫铭[1] 黄明[1] 罗卓荆[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710033 [2]解放军第359医院全军肢体伤残矫治中心,镇江212001 [3]成都军区成都总医院骨科,成都610000

出　　处：《中国矫形外科杂志》2011年第2期131-135,共5页Orthopedic Journal of China

摘　　要：[目的]观察银杏叶提取物（EGb761）对急性大鼠脊髓损伤后炎症反应及血脊髓屏障的保护作用,探讨其对急性脊髓损伤的作用机制。[方法]120只雌性SD大鼠,体重200~220 g,随机分为假手术组（A组）、损伤对照组（B组）、EGb761治疗组（C组）,每组40只。A组仅行T9椎板切除术,不致伤脊髓。B、C组用改良Allen＇s法以25gcf致伤力制作大鼠T9脊髓损伤模型,C组术后至处死前每日经腹腔给予EGb761 100 mg/kg（首次给药在术后30min）,A、B组在同一时间给予等量生理盐水。术后3、6、24、72 h分批处死动物,以T9为中心取损伤节段脊髓,采用Evans蓝含量测定法观察SCI后血脊髓屏障（blood cerebrospinal barrier,BSCB）通透性的变化,采用ELISA法测定脊髓组织白介素-1β（interleukin-1β,IL-1β）的含量,免疫组织化学方法检测细胞间粘附分子-1（intercellular adhe-sion molecular-1,ICAM-1）在脊髓组织中的表达变化。[结果]A组各时间点脊髓中无明显Evan＇s蓝渗漏,IL-1β含量及ICAM-1表达维持在基础水平;B组各时间点Evan＇s蓝渗漏、IL-1β含量及ICAM-1表达量均明显高于A组;C组EGb761干预后在6、24、72 h时Evan＇s蓝通过BSCB漏入脊髓组织量显著低于B组,在各时间点IL-1β、ICAM-1的表达均较B组明显减少。[结论]在Allen＇s大鼠急性脊髓损伤模型中,EGb761能降低局部脊髓中IL-1β含量,下调ICAM-1的表达,减轻BSCB的破坏。[Objective]To observe whether the extract of Ginkgo biloba（EGb761） has protective effect on inflammatory reaction and blood-spinal cord barrier after spinal cord injury in rat model and to study its mechanism.[Method]Totally 120 SD female rats were randomly divided into three groups,group A（sham-operated group）,group B（Allen impact SCI group） and group C（EGb761 treated group）.Each group had 40 rats for test.Rats in group A underwent laminectomy alone.The modified Allen method was adopted at the T9 level in groups B and C to induce the acute spinal cord injury.For group C,EGb761 was delivered 100 mg/kg/d intraperitoneally half an hour after injury for the first time and per day until killed.At the same time,vehicle-treated animals received physiological saline in the same volumes as in the EGb761-treated group.The rats were sacrificed at 3,6,24 and 72 h post-injury and T9 spinal cord specimen was estracted.The blood-spinal cord barrier（BSCB） of cords was determined by Evans blue content detection,interleukin-1β（IL-1β） expression was detected by ELISA method and intercellular cell adhesion molecule-1（ICAM-1） protein in spinal cord were detected by immunohistochemistry assay.[Result]In group A,there was no obvious leakage of Evans blue around the injuried site and the concentrations of IL-1β and ICAM-1 were maintained at a low level.Compared to group A,the leakage of Evans blue and the concentrations of IL-1β and ICAM-1 were significantly higher.After application of EGb761,the leakage of Evans blue were decreased significantly at all time points except 3 hours post-injury when compared to group B.Additionally,the concentrations of IL-1β and ICAM-1 were decreased significantly at each time point.[Conclusion]In Allen＇s acute SCI rat model,EGb761 could decrease the local concentration of IL-1β,suppress the abnormal expression of ICAM-1 protein and attenuate disruption of BSCB permeability.

关 键 词：脊髓损伤 EGB761 血脊髓屏障 白介素-1Β 细胞间粘附分子-1 

分 类 号：R651.2[医药卫生—外科学]