罗格列酮对经诱导的骨髓间充质干细胞ALP、BMP-2及TGF-β1分泌的影响  

Effects of rosiglitazone on the secretion of ALP, BMP-2, and TGF-β1 from rat bone mesenchymal stem cells

作　　者：舒晓春[1] 孟晓军[1] 庞天骄[1] 朱丹华[1] 叶礼红[1] 

机构地区：[1]中山大学附属第五医院内分泌科,珠海519000

出　　处：《中华内分泌代谢杂志》2011年第1期53-56,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的 体外观察罗格列酮对骨髓间充质干细胞（BMSCs）向成骨细胞分化的影响,并观察对碱性磷酸酶（ALP）、骨形成蛋白-2（BMP-2）、转化生长因子β1（TGF-β1）分泌的影响,探讨其对骨代谢的作用机制.方法 无菌条件下从大鼠长骨骨髓中分离获取BMSCs,采用全骨髓贴壁培养法对BMSCs进行纯化、传代扩增,随后在1、2、5、10 μmol/L罗格列酮干预下诱导成骨细胞分化培养21 d,进行茜素红染色观察矿化结节,并测定成骨细胞标记物ALP、BMP-2及TGF-β1的分泌.结果 1、2、5、10 μmol/L罗格列酮干预组与成骨经典组对比,成骨细胞的钙结节形成比例显著降低（P＜0.05）,ALP、BMP-2、TGF-β1水平呈剂量依赖性地下降（均P＜0.05）.结论 罗格列酮可剂量依赖性地抑制BMSCs向成骨细胞分化,这可能是罗格列酮致骨质疏松的重要机制.Objective To observe the effects of rosiglitazone on differentiation of rat bone-marrow stromal cells （BMSCs） into osteoblasts （OB） and on secretion of alkaline phosphatase （ALP）, bone morphogenetic protein2 （BMP-2）, and transforming growth factor-β1 （TGF-β1） in order to investigate its mechanism of the impact on the bone metabolism.Methods BMSCs from long bone were bred by using differential time adherent culture method,and then were interfered with 1,2,5,10 μmol/L rosiglitazone to differentiate into osteoblasts in the presence of an osteogenic medium.The rate of mineralization was examined by staining mineralized nodules with Alizarin red S,and the secretion of ALP, BMP-2, and TGF-β1 was examined by enzyme linked immunosorbent assay （ELISA）after 21 d of culture.Results Compared with the classic group, the rate of mineralization was significantly decreased by 1,2,5 and 10 μmoL/L rosiglitazone （P〈0.05）, the levels of ALP, BMP-2, and TGF-β1 decreased in a dose-depedent manner （P〈0.05）.Conclusion Rosiglitazone dose-dependently inhibits differentiation of BMSCs into osteoblasts, which may be an important mechanism in causing osteoporosis.

关 键 词：骨髓间充质干细胞 罗格列酮 成骨细胞 骨质疏松 

分 类 号：R965[医药卫生—药理学]