血管铸型技术检测组织工程多孔支架血管化的实验研究  

Experimental study on revascularization detection in tissue engineered scaffold using vascular cast technique

作　　者：武延格[1] 杨林[1] 孙学峰[1] 王正[1] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院胸外科,广东深圳518020

出　　处：《中国临床解剖学杂志》2011年第1期87-88,共2页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金资助(30500499)

摘　　要：目的探索利用血管铸型技术观察组织工程多孔支架体内再血管化情况的可行性。方法将DegraPol多孔管状支架置入SD大鼠腹腔大网膜包埋1周,应用解剖学中的血管铸型技术,观察组织工程支架体内形成新生血管的情况。结果大网膜包埋后在DegraPol支架外部发现有较多的蓝色显影微细血管环绕,在微孔支架内部,蓝色显影穿透支架并沿支架内孔分布。结论血管铸型适合用于微血管检测,可作为组织工程人工器官再血管化检测的一种新方法。Objective To explore the feasibility of vascular cast for revascularization of tissue engineered organ. Method Tissue engineered scaffolds DegraPol were implanted in rat omental 1 weeks. The anatomic technology of vascular cast was used to observe revascularization of DegraPol scaffold. Results Vascular cast showed more blue vessels around the DegraPol scaffold in omental implants. Micro-vessels were found grew into the porous scaffold. Conclusions Vascular cast technique is suitable for detecing the microvascular, and could be used as a new method to observe revascularization of tissue engineered organ.

关 键 词：血管铸型 组织工程 支架 再血管化 

分 类 号：Q819[生物学—生物工程]