矮牵牛PMADS9基因的结构特征和mRNA的表达分析  

The Gene Structure and mRNA Expression Analysis of PMADS9 from Petunia

作　　者：陈璟[1] 李名扬[1] 闫明旭[1] 郭余龙[1] 

机构地区：[1]西南大学园艺园林学院重庆市花卉工程技术研究中心,重庆400716

出　　处：《园艺学报》2011年第1期108-116,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30671481)

摘　　要：PMADS9基因是MADS-box基因AGL15亚家族成员,而AGL15基因被认为在开花植物中具有促进胚胎发生、延缓衰老进程的作用。使用PCR法克隆到了PMADS9基因的基因组序列EU338501,通过同家族基因比较分析发现矮牵牛PMADS9基因和番茄SIMBP11基因组织结构相似,和其他同源基因相比有着较长的第3外显子和庞大的1、2内含子等显著特征。通过Southern杂交初步判定其在基因组中可能为单拷贝。利用荧光定量分析发现PMADS9基因在花药和子房中优先表达,并在随后合子胚发育过程中持续表达,这与AtAGL15在拟南芥中的表达模式略有不同。对矮牵牛幼苗施加不同化学和物理处理后发现PMADS9基因对2,4-D和GA3处理有反应,并在多种胁迫环境下表达量增加,可能参与植物抗逆途径。Petunia PMADS9 gene is a member of the AGL15-like clade of MADS domain regulatory factors,and Arabidopsis AtAGL15 is considered to promote embryogenesis and repress the progression of a specific subset of age-related developmental program in the flowering plant.We cloned the genomic sequence（EU338501）of petunia PMADS9 by PCR and compared the gene structure by comparative analysis of homologous genes.The results showed that both petunia PMADS9 and tomato SIMBP11 had a long third exon and large first and second introns,which is different from other homologous genes.The Southern hybridization result showed that there may be only one copy in the genome.A real-time PCR was performed to observe the expression profiling of PMADS9.The results showed that PMADS9 preferentially expressed in anther and ovary and had low expression in other organs,which is slightly different from Arabidopsis AGL15.By using different chemical and physical treatments to the seedlings of petunia,we found PMADS9 expression responded to 2,4-D and GA3 and increased in different environment stresses which suggested it involved in plant stress resistance.

关 键 词：矮牵牛 PMADS9基因 表达模式 抗逆 

分 类 号：S681.6[农业科学—观赏园艺]