中药黄斑明目胶囊防治N-甲基-N-亚硝脲诱导大鼠视网膜变性的早期效应  

Early effects of Huangbanmingmu capsule on retinal degeneration in rats induced by N-methyl-N-nitrosourea

作　　者：王延东[1] 甄栋钦[1] 姚向超[1] 唐细兰[1] 叶成添[1] 

机构地区：[1]中山大学中山眼科中心药剂科,广州510060

出　　处：《中国临床药学杂志》2011年第1期8-12,共5页Chinese Journal of Clinical Pharmacy

基　　金：广东省中医药管理局科研项目(编号2009165)

摘　　要：目的观察中药黄斑明目胶囊对N-甲基-N-亚硝脲(MNU)诱导大鼠视网膜变性的早期拮抗效应。方法将鼠龄为43 d的♀SD大鼠30只随机分为3组,每组10只。黄斑明目胶囊组以中药黄斑明目胶囊溶解后灌胃,正常对照组和模型对照组以等量蒸馏水灌胃,bid,连续7 d。给药7d后,黄斑明目胶囊组和模型对照组大鼠予MNU 40 mg·kg^(-1),ip;正常对照组注射等量生理盐水。观察MNU处理后12、24、48、72 h各组大鼠视网膜电图(ERG)a波及b波的变化,并于MNU处理后7 d处死大鼠,取眼球做组织学观察。结果正常对照组大鼠不同时间点ERG a波及b波的振幅差异无统计学意义;模型对照组在MNU处理后ERG a波及b波的振幅均呈持续性、进行性下降;中药黄斑明目胶囊可明显抑制ERG a波及b波振幅的降低。通过透视电子显微镜和光学显微镜对视网膜的组织学观察显示,中药黄斑明目胶囊组的病理组织学损害较模型对照组明显减轻。结论中药黄斑明目胶囊可拮抗MNU诱导的视网膜变性大鼠的早期损伤。AIM To observe the early antagonistic effects of Huangbanmingmu capsule on retinal degeneration induced by N-methyl-N-nitrosourea（MNU） in SD rats.METHODS Female SD rats of 43-day-old were randomly divided into control group,model control group and Huangbanmingmu capsule group.Huangbanmingmu capsule was irrigated into rats with intragastric administration in Huangbanmingmu capsule group for continuous 7 d twice one day.Control group and model group were given equal distilled water.SD rats in 7 d after treatment received a single injection of 40 mg·kg^（-1） MNU,ip in model group and Huangbanmingmu capsule group;while control group was given equal normal saline. Changes of a wave and b wave of electroretinogram（ERG） were observed in 12,24,48 and 72 h after MNU injection. The rats were killed in 7 d after MNU treatment and histopathologic changes were observed.RESULTS The difference of the average amplitude of ERG a wave and b wave was not significant between different time in control group.The average amplitude of ERG a wave and b wave gradually decreased after MNU treatment in model group.Huangbanmingmu capsule could evidently inhibit lose of a wave and b wave.The histopathologic injury of rat retina in Huangbanmingmu capsule group was lighter than in model control group by means of transmission electron microscopy and light microscopy.CONCLUSION Huangbanmingmu capsule has early antagonistic effects on retinal degeneration induced by MNU in SD rats.

关 键 词：黄斑明目胶囊 视网膜变性 N-甲基-N-亚硝脲 拮抗效应 

分 类 号：R285.5[医药卫生—中药学]