清肝化瘀合剂质量标准的建立  

Establishment of quality standard for Qingganhuayu mistura

作　　者：孙梁燕[1] 许永军[1] 杨文清[1] 

机构地区：[1]浙江省慈溪市人民医院药剂科,慈溪315300

出　　处：《中国临床药学杂志》2011年第1期38-42,共5页Chinese Journal of Clinical Pharmacy

基　　金：浙江省慈溪市科技计划项目(编号CR200710)

摘　　要：目的建立清肝化瘀合剂的质量标准。方法采用薄层色谱法鉴别处方中的主要药味绞股蓝、山楂、赤芍、白芍、丹参、川芎;采用Hypersil 0DS_2(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.8%磷酸水溶液-水(16:11:73,V/V/V),流速0.8 mL·min^(-1),检测波长230 nm,柱温25℃。结果主要药味具有鉴别特征;芍药苷进样量在16.5～82.5 mg·L^(-1)时与峰面积之间的线性关系为y=29.9794x-40.2444,r=0.999 9,平均加样回收率为98.89%,RSD为1.60%。结论该法简便准确,可作为该制剂质量控制的标准方法。AIM To establish the quality standard of Qingganhuayu mistura.METHODS The thin layer chromatography （TLC） method was applied to identify the Gynostemma pentaphyllum（Thunb.） Makino,Crataegus pinnatifida Bge,Paeonia lactiflora Pall,Salvia miltiorrhiza Bge and Ligusticum chuanxiong Hort.The column was Hypersil ODS_2（250 mm×4.6 mm,5μm）,the mobile phase was acetonitrile - 0.8%phosphoric acid solution-water（16：11：73, V/V/V）,the flow rate was 0.8 mL·min^（-1）,the detective wavelength was 230 nm and the column temperature was 25℃. RESULTS The chief medicinal crops could be identified by TLC.The calibration curve of paeoniflorin was linear in the range of 16.5 - 82.5 mg·L^-1 and its regressive equations was y = 29.979 4x - 40.244 4（r = 0.999 9）,the average recovery was 98.89%,and the RSD was 1.60%.CONCLUSION The method is simple,accurate and can be used as a quality standard for Qingganhuayu mistura.

关 键 词：清肝化瘀合剂 薄层色谱法 高效液相色谱法 芍药苷 

分 类 号：R286[医药卫生—中药学]