力竭游泳运动后大鼠伏核内NF-κB、cAMP的表达  

Effects of exhausted swimming styles on the expression of NF-κB and cAMP in nucleus accumbens of rat

作　　者：范琳琳[1] 夏仲年[1] 杨成志[1] 和荣丽[1] 何生[1] 成晓龙[1] 杨桂姣[1] 

机构地区：[1]山西医科大学人体解剖学教研室,太原030001

出　　处：《神经解剖学杂志》2011年第1期72-76,共5页Chinese Journal of Neuroanatomy

基　　金：山西医科大学科技创新基金(01200803)

摘　　要：目的:观察力竭游泳运动后大鼠伏核(NAc)内核转录因子-κB(NF-κB)和环腺苷酸(cAMP)的表达。方法:成年雄性SD大鼠随机分为对照组和力竭组。建立一周力竭游泳运动模型后,按0、4、8、12、16、20、24 h七个时间点,采用免疫印迹和免疫组织化学技术观察NF-κB在伏核内的表达变化,应用酶联免疫吸附技术检测伏核内cAMP的含量变化,并与对照组进行比较。结果:对照组伏核内NF-κB的表达较少,cAMP含量较低,力竭组NF-κB的表达明显增多,cAMP含量也明显增加,两者均于8 h达到较高水平,随后维持在较高水平,于16 h后开始回落,24 h已明显下降。结论:力竭游泳训练1周可以使大鼠伏核内NF-κB与cAMP得到有效的活化,且两者的表达变化具有明显相同时效性,提示它们可能具有协同效应。Objective：To observe the expression of NF-κB and cAMP in nucleus accumbens（NAc） of adult rats after exhausted swimming.Methods：Adult male SD rats were divided into the control group and exhausted group.Taking 0,4,8,12,16,20 and 24 hours after exhausted swimming for a week,Western Blot and immunohistochemical methods were used to detected the change of NF-κB in NAc,ELISA was used to examine the expressional change of cAMP.All results were combined with control group.Results：The expression of NF-κB and cAMP were extremely lower in control group,but increased in NAc after exercise,both reach higher levels at 8 h,then retainned higher levels for a moment,fell after 16 h and descended 24 h later dramatically.Conclusion：NF-κB and cAMP can be activated effectively in NAc after exhausted exercise,and the tendencys of change of them are similar.It is suggested that they have synergistic effect.

关 键 词：NF-ΚB CAMP 游泳运动 伏核 大鼠 

分 类 号：R87[医药卫生—运动医学]