复方中药安胃汤对慢性萎缩性胃炎大鼠模型TFF mRNA表达的影响  

Effect of treatment with Anwei Decoction on TFF mRNA expression in rats with chronic atrophic gastritis

作　　者：林寿宁[1] 韦维[2] 黄贵华[3] 朱永苹[1] 刘伟峰[1] 万小刚[1] 朱光辉[1] 张倩[1] 

机构地区：[1]广西中医学院瑞康医院消化内科,广西壮族自治区南宁市530011 [2]广西中医学院,广西壮族自治区南宁市530001 [3]广西中医学院一附院消化内科,广西壮族自治区南宁市530022

出　　处：《世界华人消化杂志》2011年第1期68-73,共6页World Chinese Journal of Digestology

基　　金：国家科技支撑计划(十一五)项目子课题基金资助项目;No.2007BAIO7A17-02;广西自然科学基金资助项目;No.桂科自0728187~~

摘　　要：目的:探讨复方中药安胃汤对实验性慢性萎缩性胃炎大鼠模型的TFFmRNA表达的影响.方法:采用甲基硝基亚硝基胍(MNNG)慢性萎缩性胃炎大鼠模型,48只清洁级健康♂Wistar大鼠随机分为空白组、病理模型组、复方中药安胃汤组、胃复春对照组共3组.实时荧光定量PCR方法观察复方中药安胃汤对实验性慢性萎缩性胃炎大鼠模型胃黏膜TFF1、TFF2、TFF3mRNA表达的影响,对后者进行图像分析及统计学处理.结果:与空白组相比,病理模型组、复方中药安胃汤组和胃复春对照组TFF1mRNAβ-actin值均显著提高(A值:0.53±0.10,0.86±0.13,0.83±0.10vs0.39±0.14,均P<0.01),复方中药安胃汤组高于胃复春对照组(P<0.01).与病理模型组相比,复方中药安胃汤组和胃复春对照组TFF2mRNAβ-actin值均显著提高(A值:0.90±0.06,0.72±0.10vs0.35±0.06,均P<0.01),复方中药安胃汤组高于胃复春对照组(P<0.05).与病理模型组相比,空白组、复方中药安胃汤组和胃复春对照组TFF3mRNAβ-actin值均显著降低(A值:0.25±0.10,0.43±0.04,0.64±0.04vs0.95±0.23,均P<0.01),复方中药安胃汤组低于胃复春对照组(P<0.05).结论:复方中药安胃汤可能通过增加胃黏膜TFF1、TFF2mRNA表达,降低胃黏膜TFF3mRNA表达而起到治疗慢性萎缩性胃炎作用.AIM： To investigate the effect of treatment with Anwei Decoction （AD） on the expression of trefoil factor （TFF） mRNAs in rats with chronic atrophic gastritis （CAG）. METHODS： Forty eight healthy rats were randomly divided into 4 groups： normal control group, pathologic model group, Anwei Decoction group, and Weifuchun group. CAG was in- duced in rats with N-methy-N-nitro-N-nitroguanidine （MNNG）. The expression of TFF mRNAs in rat gastric mucosa was determined by realtime polymerase chain reaction （PCR）. RESULTS： Compared with the normal control group, the expression of TFF1 mRNA was significantly enhanced in the pathologic model group, Anwei Decoction and Weifuchun groups （0.53 ± 0.10, 0.86 ± 0.13, 0.83 ± 0.10 vs 0.39 ± 0.14; all P 0.05）. The expression level of TFF1 mRNA was significantly higher in the Anwei Decoction group than in the Weifuchun group （P 0.01）. Compared with the pathologic model group, the expression of TFF2 mRNA was also signifi cantly enhanced in Anwei Decoction and Weifuchun groups （0.90 ± 0.06, 0.72 ± 0.10 vs 0.35 ± 0.06, both P 0.05）. The expression level of TFF2 mRNA was significantly higher in the Anwei Decoction group than in the Weifuchun group （P 0.05）. In contrast, compared with the pathologic model group, the expression of TFF3 mRNA was remarkably reduced in the normal control group, Anwei Decoction and Weifuchun groups （0.25 ± 0.10, 0.43 ± 0.04, 0.64 ± 0.04 vs 0.95 ± 0.23, all P 0.05）. The expression level of TFF3 mRNA was significantly lower in the Anwei Decoction group than in the Weifuchun group （P 0.05）. CONCLUSION： Anwei Decoction exerts a therapeutic effect against CAG possibly by enhancing the expression of TFF1 and TFF2mRNAs and reducing the expression of TFF3 mRNA in gastric mucosa.

关 键 词：安胃汤 慢性萎缩性胃炎 三叶肽家族 

分 类 号：R259[医药卫生—中西医结合]