Karmali和Columbia培养基初次分离幽门螺杆菌效果的比较  

Comparison of Karmali agar medium and Columbia agar medium for isolation of Helicobacter pylori from gastric biopsy specimens

作　　者：何利华[1] 张茂俊[1] 刘国栋[1] 张慧芳[1] 乔博[1] 张建中[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病诊断室,北京市102206

出　　处：《世界华人消化杂志》2011年第1期98-101,共4页World Chinese Journal of Digestology

基　　金：国家科技支撑计划基金资助项目;No.2007BAI04B02~~

摘　　要：目的:比较两种基础培养基对幽门螺杆菌(H.pylori)感染者的胃黏膜标本初次分离率的差异.方法:将168份13C呼气试验阳性患者的胃黏膜标本研磨后,等量接种于Karmali和Columbia血琼脂培养基,置于37℃微需氧环境(50mLLO2,100mLLCO2和850mLLN2),分别培养24、48、72、96h,观察细菌的生长情况、进行菌落鉴定并比较H.pylori阳性率的差异.结果:培养24h,Karmali和Columbia培养基上H.pylori阳性检出率分别为9.5%和7.1%,两者之间没有显著差异(P=0.125);培养48、72和96h后,Karmali培养基的检出率显著高于Columbia培养基(82.1%vs45.8%,94.6%vs79.2%,97.0%vs88.7%,P<0.05).结论:Karmali培养基对H.pylori初次分离的效果明显优于Columbia培养基.Karmali培养基是一种有效的H.pylori初次分离培养的培养基.AIM： To compare the isolation rates of the Helicobacter pylori （H.pylori） from gastric biopsy specimens achieved using Karmali and Columbia agar media. METHODS： Gastric biopsy specimens were collected from 168 H.pylori-positive patients and cultivated on Karmali and Columbia agar plates under microaerophilic conditions （5% O2, 10% CO2, and 85% N2）. Single colonies of H.pylori were identified by biochemical test and PCR. The isolation rates of H.pylori achieved using the two types of media at 24, 48, 72, and 96 h werecompared. RESULTS： There was no significant difference in the isolation rate between Karmali and Columbia agar at 24 h （P = 0.125）. In contrast, the isolation rates achieved using Karmali agar at 48, 72 and 96 h were signif icantly higher than those using Columbia agar （82.1% vs 45.8%, 94.6% vs 79.2%, 97.0% vs 88.7%, all P 0.05）. CONCLUSION： Higher isolation rate of H.pylori from gastric biopsy specimens was achieved with Karmali agar than with Columbia agar. Karmali agar can be used as an effective medium for isolation of H.pylori from gastric biopsy specimens.

关 键 词：幽门螺杆菌 固体培养基 胃黏膜标本 分离培养 

分 类 号：R446.5[医药卫生—诊断学]