共刺激分子在复感儿中医分型中的表达研究  

Clinical Significance of Costimulatory Molecule Expression in Syndrome Differentiation of Recurrent Respiratory Tract Infection Children

作　　者：章文平[1] 廖永州[1] 何胜晓[1] 李宜瑞[1] 

机构地区：[1]广州中医药大学第一附属医院儿科,广东广州510405

出　　处：《广州中医药大学学报》2011年第1期17-21,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局科技项目(编号:20070432)

摘　　要：【目的】通过观察反复呼吸道感染(RRTI)非急性感染期肺脾气虚、肺脾气阴两虚型患儿(简称"复感儿")T细胞CD8+CD+28的表达,探讨两个证型T淋巴细胞亚群的表达特点及临床意义,为RRTI的中医辨证提供可能的物质基础及客观依据。【方法】选择RRTI非急性期肺脾气虚、肺脾气阴两虚患儿各30例,运用流式细胞仪对RRTI患儿外周血T淋巴细胞亚群表达进行检测,并与正常对照组30例比较,结果运用SPSS/PC10.0统计软件包进行分析处理。【结果】与正常对照组比较,RRTI的CD8+、CD28+、CD8+CD28+%、CD8+CD28+表达降低,而CD8+CD28-%、CD8+CD28-表达增高。RRTI不同中医分型T细胞亚群的表达也不同。经多元回归分析,中医分型证候积分与CD8+CD28+%、CD8+CD28-%的表达相关。【结论】T淋巴细胞亚群失衡为RRTI发病机制之一,且与RRTI非急性感染期肺脾气虚证与肺脾气阴两虚证的证候积分相关,这可能将有助于中医辨证分型。Objective To observe T lymphocytes CD8＋ CD28＋ expression in recurrent respiratory tract infection （RRTI） children with the syndromes of lung-spleen Qi deficiency and lung-spleen Qi-yin deficiency during the non-acute infective stage, and to investigate the relationship of T lymphocytes subtypes distribution with syndrome differentiation of RRTI. Methods Peripheral T lymphocyte CD8＋ CD28＋ expression was detected by flow cytoinetry in 30 RRTI children with lung-spleen Qi deficiency and in 30 RRTI children with lung-spleen Qi-yin deficiency. The results were compared with those in 30 healthy children, and then were analyzed with SPSS/PC10.0 software. Results Compared with the healthy children, the percentages of CD8＋ , CD2＋ and CD8＋ CD28＋ as well as CD8＋ CD28＋ expression were decreased, while CD8＋ CD28 percentage and CD8＋CD2＋ expression were increased. The distribution of T lymphocytes subtypes varied in RRTI with different syndrome patterns. The results of multiple regression analysis showed that the scores of syndromes were con＇elated with the percentages of CD8＋ CD28＋ and CD8＋ CD28＋. Conclusion The imbalance of the distribution of T lymphocyte subtypes is one of the pathogenesis of RRTI. The expression of T lymphocyte subtypes is correlated with the syndrome scores of RRTI children with the syndromes of lung-spleen Qi deficiency and lung-spleen Qi-yin deficiency during the non-acute infective stage, which will be helpful for the syndrome differentiation of RRTI in children.

关 键 词：反复呼吸道感染/中医病机 肺脾气虚 肺脾气阴两虚 T淋巴细胞亚群 儿童 

分 类 号：R272[医药卫生—中医儿科学]