雌激素相关受体α特异性抑制剂XCT-790对成骨细胞株MG-63增殖分化的影响  

Effect of Estrogen-related Receptor-alpha Specific Antagonist XCT-790 on Proliferation and Differentiation of Osteoblast-like Cell Line MG-63

作　　者：杨冰 谭彤燕 梁笃 曾展鹏[3] 周驰[3] 王海彬[3] 

机构地区：[1]广州市正骨医院 [2]95100部队 [3]广州中医药大学第一附属医院骨科

出　　处：《广州中医药大学学报》2011年第1期43-45,48,105,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:30973761);广东省自然基金资助项目(编号:8151040701000016);广东省中医药管理局基金资助项目(编号:2009306;2009216)

摘　　要：【目的】探讨雌激素相关受体α(ERRα)特异性抑制剂XCT-790对成骨细胞增殖分化的影响。【方法】采用CCK-8试剂检测XCT-790对细胞活力的影响,茜素红染色检测XCT-790对成骨细胞矿化能力的影响,流式细胞仪检测XCT-790对细胞内游离钙离子浓度的影响。【结果】XCT-790可显著降低成骨细胞的活力(P<0.05或P<0.01),对细胞前期分化标志碱性磷酸酶(ALP)活性无显著影响(P>0.05),但可显著降低细胞的矿化能力,显著提高细胞内钙离子浓度(P<0.01)。【结论】抑制ERRα的活性可以降低成骨细胞的增殖和矿化能力而对ALP的活性无影响。Objective To study the effect of estrogen-related receptor-alpha （ERRor） specific antagonist XCT-790 on the proliferation and differentiation of osteoblast. Methods Cell activities were detected by CCK-8 kit, the effect of XCT-790 on the osteoblast mineralization was detected by alizarin red staining method, and the effect of XCT-790 on the cellular free calcium was detected by flow cytometry. Results ERRα specific antagonist XCT-790 could obviously inhibit cell viabilities （ P 〈 0.05 or P 〈 0. 01 ） , increase the cellular free calcium concentration （ P 〈0.01）, and decrease osteoblast mineralization, while had no effect on the activities of alkaline phosphatase （ALP）, which is the identification marker of early cell differentiation. Conclusion The inhibition of ERRα activity can result in the decrease of osteoblast proliferation and mineralization but has no effect on ALP activities.

关 键 词：XCT-790/药物作用 成骨细胞/病理学 细胞增殖 细胞培养 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]