用酵母双杂合系统筛选与人血小板生成素受体c-Mpl相互作用的蛋白质的初报  

Preliminary Study of Proteins that Interact with Human Thrombopoietin Receptor c-Mpl Screened by Yeast Two-Hybrid System

作　　者：周伟国[1] 冯丽冰[1] 赵新燕[1] 戴卫列[1] 李昌本[1] 赵寿元[1] 

机构地区：[1]遗传工程国家重点实验室

出　　处：《复旦学报（自然科学版）》1999年第5期502-505,共4页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金;国家"八六三"计划生物技术领域青年基金

摘　　要：血小板生成素（ｔｈｒｏｍ ｂｏｐｏｉｅｔｉｎ， ＴＰＯ）是调节血小板生成最主要的细胞因子，其生物学效应由其受体ｃ－Ｍｐｌ介导．利用酵母双杂合系统（ｔｗ ｏ－ｈｙｂｒｉｄ ｓｙｓｔｅｍ ）筛选与ｃ－Ｍｐｌ相互作用的蛋白质因子，以Ｇａｌ４ ＢＤ融合ｃ－Ｍｐｌ膜内部分ｃＤＮＡ 的ｐＡＳＭＭ 为靶蛋白质粒，筛选了人胎盘ｃＤＮＡ 文库，分离到人波形纤维蛋白（ｖｉｍ ｅｎｔｉｎ）的部分编码序列，首次检测到波形纤维蛋白与ＴＰＯ 受体之间的相互作用，这提示细胞骨架蛋白可能在ＴＰＯThrombopoietin (TPO) is the major cytokine involved in platelet production and exerts its effects via the receptor c Mpl. The yeast two hybrid system has been used to screen the proteins interacting with c Mpl. The plasmid pASMM containing Gal 4 DNA binding domain fused with c Mpl intracellular domain was used as a target plasmid to screen a human placenta cDNA library, a part of coding sequence of vimentin was obtained. Therefore, it is the first time to detect an interaction between vimentin and TPO receptor. The results suggest that this cytoskeletal protein may play an important role in TPO signal transduction pathway.

关 键 词：血小板 生成素受体 酵母双杂合系统 蛋白质 

分 类 号：Q26[生物学—细胞生物学] R331.14[医药卫生—人体生理学]