CD44v6和CEA-mRNA定量检测在恶性胸腔积液鉴别诊断中的应用  

The quantitative determination of CD44v6 and CEA-mRNA in differenctial diagnosis of malignant pleural effusion

作　　者：蒙佳莲 

机构地区：[1]广西南宁市邕宁区人民医院检验科,广西南宁530200

出　　处：《右江民族医学院学报》2011年第1期13-15,共3页Journal of Youjiang Medical University for Nationalities

摘　　要：目的探讨联合检测细胞表面黏附分子(CD44v6)和癌胚抗原信使核糖核酸(CEA-mRNA)在恶性胸腔积液鉴别诊断中的应用价值。方法选取90例胸腔积液患者,其中良性疾病组患者39例,恶性疾病组患者51例。采用ELISA法检测胸腔积液CD44v6含量,荧光定量聚合酶链反应(FQ-PCR)方法检测恶性胸腔积液CEA-mRNA,并比较这两种物质在良、恶性胸腔积液中的阳性率。结果 CD44v6和CEA-mRNA在恶性胸腔积液组中阳性率明显高于良性胸腔积液组,联合检测敏感性和特异性高于单项检测。结论 CD44v6被认为是肿瘤的特征性和早期指标,与肿瘤的生长、侵袭和转移有关;CEA-mRNA几乎只存在于某些肿瘤细胞内,细胞之外不能存在游离的CEA-mRNA,在胸腔积液内检测到CEA-mRNA,提示胸腔内发生了能表达CEA-mRNA的肿瘤细胞的胸腔转移。两者联合检测,对于诊断和鉴别胸腔积液的性质有重要的临床意义。Objective To evaluate the clinical values of determintion of the cell surface adhesion molecules （CD44V6） in combination with carcinoembryonic antigen messengemer ribonucleie acid （CEA-mRNA） in differenetial diagnosis of malignant pleural effusion. Methods Chose 90 patients with pleural effusion, of which 39 patients with benign diseases, 51 patients with malignant diseases. An ELISA method was used to detect the quantitation of CLM4v6, and a FQ - PCR method was used to detect CEA- mRNA in malignant pleural effusion. And com- pared of the positiveexpression rates of these two in benign and malignant pleural effusion. Results The positive - expreesion rate of CD44v6 and CEA- mRNA in malignant pleural effusion group was higher than that in the benign pleural effusion group. The sensitivity and specifity in the combination detection were higher than those in single detection. Conclusion CD44v6 was considered as early marker and specifity of tumor. Which was considered as genesis, invasiveness and metastasis of tumor. Ahnost all of the CEA- mRNA remains in certain tumor cells. Out of the tumor cells can not have CEA- mRNA. The CEA - mRNA can be measured in pleural effusion＇ which indicated that the pleural metastasis of CEA- mRNA tumor cells. The clinical significance of the incombination of these two can differentiate and diagnose of the quality of pleural effusion.

关 键 词：CD44V6 CEA—mRNA 胸腔积液 

分 类 号：R446.19[医药卫生—诊断学]