白首乌中C_(21)甾总苷盐酸水解条件的优化  

Study on Optimization of the Hydrolysis Process of C_(21) Steroidal Glycoside of Bai Shou Wu by Hydrochloric Acid

作　　者：赵鑫[1,2] 沈舒[1] 李友宾[2] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]江苏省中医药研究院,江苏南京210028

出　　处：《现代中药研究与实践》2011年第1期39-41,共3页Research and Practice on Chinese Medicines

基　　金：江苏省自然科学基金(BK2008488);江苏省自然科学基金(BK2009457)

摘　　要：目的研究白首乌C21甾总苷盐酸水解的最优水解条件。方法采用正交试验法,考察水解时间、水解温度、盐酸浓度等因素对告达庭-3-O-β-D-磁麻糖苷含量的影响。结果影响盐酸水解的最优水解条件为:0.5%盐酸溶液,60℃加热回流5h。结论优化的水解条件能明显提高白首乌总苷中有效成分的含量,对进一步研究白首乌总苷抗肿瘤活性的增强具有一定意义。Objective To study the optimization of hydrolysis process of C21 steroidal glycoside of Bai Shou Wu by hydrochloric acid.Methods The effects of hydrochloric acid concentration,reaction temperature and reaction time had been investigated using orthogonal design with caudatin-3-O-β-D-cymaropyranosyl content as the index.Results The optimum hydrolysis condition was as follows： refluxing for 5 h with 50 mL of 0.5% dilute HCl solution at 60 ℃.Conclusion The content of active ingredients in general steroidal glycosides of Bai Shou Wu under the optimum hydrolysis condition is raised obviously and has a great part in improving anti-tumor activity.

关 键 词：白首乌C21甾总苷 盐酸水解 告达庭-3-O-β-D-磁麻糖苷 

分 类 号：R284.2[医药卫生—中药学]